Agrawal Ankita, Acharya Aishwarya B, Sahu Bijayalaxmi, Barik Tapan Kumar, Patel Amiya Kumar
Department of Biotechnology and Bioinformatics, Sambalpur University, Jyoti Vihar, Burla, Sambalpur, Odisha, 768019, India.
Department of Zoology, Berhampur University, Bhanja Bihar, Berhampur, Ganjam, Odisha, 760007, India.
Int Microbiol. 2025 Jun 20. doi: 10.1007/s10123-025-00688-x.
Malaria remains the deadliest vector-borne disease globally. Among different malaria vectors, Anopheles subpictus has been regarded as the potent vector across many Indian states. Microbiota in mosquitoes are critical determinants for their development, physiology, and vector competence, which differ significantly among mosquito species, life stages, and environmental parameters. Microbial profiles linked with various life stages of the emerging malaria vector, An. subpictus remain unexplored. In this study, 16S rRNA amplicon sequencing (V1-V9 regions) was used to explore and analyze the microbial community dynamics within and between life stages. A total of 287,077 bacterial reads were generated and distributed into 40 phyla, 75 classes, 160 orders, 334 families, 961 genera, and 2398 species. Diversity analyses revealed that bacterial community structure varied significantly across the three life phases (larvae, pupae, adult) of An. subpictus. The larval stage has higher species richness compared to subsequent developmental stages. The sequence-based taxonomic profiling revealed that Methylobacterium, Clostridium, Bradyrhizobium, Cytophaga, Roseateles, Mitsuaria, Sphingomonas and Wolbachia were the dominant bacterial genera across the different life stages. Moreover, Methylobacterium sp. (51.6%) was the most abundant taxa in the adult female stage, whereas the larval stage was mainly dominated by Cytophaga (15.2%). This research advances our understanding of bacterial community composition and dynamics across the developmental succession of An. subpictus. The finding pinpoints dominant bacterial candidates that could be harnessed to develop microbial-based control strategies aimed at limiting mosquito-borne diseases.
疟疾仍然是全球最致命的媒介传播疾病。在不同的疟疾媒介中，伪斑按蚊在印度的许多邦都被视为主要媒介。蚊子体内的微生物群是其发育、生理和媒介能力的关键决定因素，这些因素在不同蚊种、生命阶段和环境参数之间存在显著差异。与新兴疟疾媒介伪斑按蚊的各个生命阶段相关的微生物特征仍未得到探索。在本研究中，使用16S rRNA扩增子测序（V1-V9区域）来探索和分析不同生命阶段内和不同生命阶段之间的微生物群落动态。总共产生了287,077条细菌读数，并将其分类到40个门、75个纲、160个目、334个科、961个属和2398个物种中。多样性分析表明，伪斑按蚊的三个生命阶段（幼虫、蛹、成虫）的细菌群落结构存在显著差异。幼虫阶段的物种丰富度高于后续发育阶段。基于序列的分类分析表明，甲基杆菌属、梭菌属、慢生根瘤菌属、噬纤维菌属、玫瑰单胞菌属、水栖菌属、鞘氨醇单胞菌属和沃尔巴克氏体属是不同生命阶段的主要细菌属。此外，甲基杆菌属（51.6%）是成年雌性阶段中最丰富的分类群，而幼虫阶段主要由噬纤维菌属（15.2%）主导。这项研究增进了我们对伪斑按蚊发育过程中细菌群落组成和动态的理解。该发现确定了主要的细菌候选对象，可利用这些对象开发基于微生物的控制策略，以限制蚊媒疾病。
