Hanisch F G, Uhlenbruck G, Egge H, Peter-Katalinić J
Abt. Immunbiologie, Universitätsklinik Köln.
Biol Chem Hoppe Seyler. 1989 Jan;370(1):21-6. doi: 10.1515/bchm3.1989.370.1.21.
Mucus glycoproteins from bovine seminal plasma were demonstrated to express the MAbs CC49- and B72.3-defined epitopes on TAG 72 antigen. Inhibition studies with reductively cleaved mucin O-glycans from bovine seminal plasma and submaxillary glands revealed that CC49 binds specifically to a core-type sialyl-oligosaccharide alditol (fraction 2c), which could be defined with regard to its primary structure by FAB- and EI-mass spectrometry combined with methylation analysis: (formula; see text) Structurally related alditols NeuAc alpha(2-6)-GalNAc-ol, Gal beta(1-3)GalNAc-ol, NeuAc alpha(2-3)-Gal beta(1-3) [NeuAc alpha(2-6)] GalNAc-ol, GlcNAc beta(1-3) [NeuAc alpha(2-6)] GalNAc-ol or NeuAc alpha(2-3) Gal beta(1-3)GalNAc-ol were not inhibitory.
已证明来自牛精浆的黏液糖蛋白在TAG 72抗原上表达单克隆抗体CC49和B72.3所定义的表位。用来自牛精浆和下颌下腺的经还原裂解的黏蛋白O-聚糖进行的抑制研究表明，CC49特异性结合一种核心型唾液酸化低聚糖糖醇（组分2c），通过快原子轰击和电子轰击质谱结合甲基化分析可确定其一级结构：（分子式；见正文）结构相关的糖醇NeuAcα(2-6)-GalNAc-ol、Galβ(1-3)GalNAc-ol、NeuAcα(2-3)-Galβ(1-3)[NeuAcα(2-6)]GalNAc-ol、GlcNAcβ(1-3)[NeuAcα(2-6)]GalNAc-ol或NeuAcα(2-3)Galβ(1-3)GalNAc-ol无抑制作用。
