Kaplan N, Rosenberg E, Jann B, Jann K
Eur J Biochem. 1985 Oct 15;152(2):453-8. doi: 10.1111/j.1432-1033.1985.tb09218.x.
Compositional analysis of the intact and carboxyl-reduced capsular polysaccharide of Acinetobacter calcoaceticus BD4 (PS-4) showed it to consist of L-rhamnose, D-glucose, D-glucuronic and D-mannose in molar ratios of 4:1:1:1. 13C-nuclear magnetic resonance spectroscopy, methylation analysis, oligosaccharide analysis and base-catalyzed beta-elimination were used to elucidate the primary structure. Oligosaccharides were obtained by enzymatic depolymerization with a specific bacteriophage-induced depolymerase and by partial acid hydrolysis. Form the results it is concluded that PS-4 consists of repeating units of the heptasaccharide (Formula: see text). The bacteriophage-induced depolymerase was found to be an endo-beta-D-glucosidase that hydrolyzed the bond beta-D-Glc-(1----3)-L-Rha to generate a heptasaccharide in 40% yield.
对醋酸钙不动杆菌BD4（PS - 4）完整的和羧基还原的荚膜多糖进行的组成分析表明，其由L - 鼠李糖、D - 葡萄糖、D - 葡萄糖醛酸和D - 甘露糖组成，摩尔比为4:1:1:1。采用13C - 核磁共振光谱、甲基化分析、寡糖分析和碱催化β - 消除法来阐明其一级结构。寡糖通过用特定噬菌体诱导的解聚酶进行酶促解聚以及部分酸水解获得。从结果得出结论，PS - 4由七糖重复单元组成（分子式：见正文）。发现噬菌体诱导的解聚酶是一种内切β - D - 葡萄糖苷酶，它水解β - D - Glc - (1----3)-L - Rha键，以40%的产率生成七糖。
