Talice Shani, Barkan Shany K, Snyder Grace A, Kozlovski Itamar, Sharoni Ton, Levy Tom, Lewandowska Magda, Ehrens David, Sultan Eliya, Azarov Alona, Oisher Shelly, Yannai Ronnie, Aharoni Reuven, Hadad Uzi, Davis Claytus, Moran Yehu, Gershoni-Yahalom Orly, Traylor-Knowles Nikki, Rosental Benyamin
The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Center for Regenerative Medicine and Stem Cells, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel; The Goldman Sonnenfeldt School of Sustainability and Climate Change, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel.
The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Center for Regenerative Medicine and Stem Cells, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel; Rosenstiel School of Marine, Atmospheric, and Earth Science, University of Miami, Miami, FL 33149, USA.
STAR Protoc. 2025 Jun 20;6(3):103913. doi: 10.1016/j.xpro.2025.103913.
Stem cell transplantation is a promising strategy for regenerating damaged tissues in Hexacorallia, a cnidarian subclass that includes stony corals threatened by anthropogenic stressors. Here, we present a protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. We describe steps for chemotherapy treatment, cell dissociation, and transplantation. We then detail procedures for assessment via fluorescence microscopy and flow cytometry. This technique lays the groundwork for potential coral restoration and conservation applications. For complete details on the use and execution of this protocol, please refer to Talice et al..
干细胞移植是一种很有前景的策略，可用于六放珊瑚纲受损组织的再生。六放珊瑚纲是刺胞动物亚纲的一个类别，其中包括受到人为压力源威胁的石珊瑚。在这里，我们展示了一种在六放珊瑚模型——星状海葵中进行细胞移植的方案。我们描述了化疗处理、细胞解离和移植的步骤。然后详细介绍了通过荧光显微镜和流式细胞术进行评估的程序。这项技术为潜在的珊瑚恢复和保护应用奠定了基础。有关该方案的使用和实施的完整详细信息，请参考塔利塞等人的研究。
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