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大气压毛细管等离子体射流非常适合为等离子体驱动的生物催化提供羟基自由基。

The Atmospheric Pressure Capillary Plasma Jet Is Well-Suited to Supply HO for Plasma-Driven Biocatalysis.

作者信息

Dirks Tim, Stoesser Davina, Schüttler Steffen, Hollmann Frank, Golda Judith, Bandow Julia E

机构信息

Applied Microbiology, Faculty of Biology and Biotechnology, Ruhr University Bochum, 44801, Bochum, Germany.

Plasma Interface Physics, Faculty of Physics and Astronomy, Ruhr University Bochum, 44801, Bochum, Germany.

出版信息

ChemistryOpen. 2025 Sep;14(9):e202500057. doi: 10.1002/open.202500057. Epub 2025 Jun 22.

DOI:10.1002/open.202500057
PMID:40545844
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12409826/
Abstract

Plasma-generated HO can be used to fuel biocatalytic reactions that require HO as a cosubstrate, such as the conversion of ethylbenzene to (R)-1-phenylethanol ((R)-1-PhOl) catalyzed by unspecific peroxygenase from Agrocybe aegerita (rAaeUPO). Immobilization is recently shown to protect biocatalysts from inactivation by highly reactive plasma-produced species; however, HO supply by the employed plasma sources (μAPPJ and DBD) is limiting for rAaeUPO performance. This study evaluates a recently introduced capillary plasma jet for suitability to supply HO in situ. HO production is modulated by varying the water concentration in the feed gas, providing a greater operating window for applications in plasma-driven biocatalysis. In a static system after 80 min of biocatalysis, a turnover number of 44,199 mol mol is achieved without significant enzyme inactivation. By exchanging the reaction solution every 5 min, a total product yield of 122 μmol (R)-1-PhOl is achieved in 700 min run time, resulting in a total turnover number of 174,209 mol mol . This study concludes that the capillary plasma jet, due to its flexibility regarding feed gas, admixtures, and power input, is well suited for in situ HO generation for plasma-driven biocatalysis tailoring to enzymes with high HO turnover.

摘要

等离子体产生的羟基自由基（HO）可用于为需要HO作为共底物的生物催化反应提供燃料，例如由高大环柄菇（Agrocybe aegerita）的非特异性过氧合酶（rAaeUPO）催化乙苯转化为（R）-1-苯乙醇（（R）-1-PhOl）。最近的研究表明，固定化可以保护生物催化剂不被高活性的等离子体产生的物质灭活；然而，所使用的等离子体源（μAPPJ和DBD）提供的HO对rAaeUPO的性能有限制。本研究评估了一种最近引入的毛细管等离子体射流用于原位供应HO的适用性。通过改变进料气体中的水浓度来调节HO的产生，为等离子体驱动的生物催化应用提供了更大的操作窗口。在静态系统中进行80分钟的生物催化后，实现了44,199 mol/mol的周转数，且酶没有明显失活。通过每5分钟更换一次反应溶液，在700分钟的运行时间内实现了122 μmol（R）-1-PhOl的总产物产量，总周转数为174,209 mol/mol。本研究得出结论，毛细管等离子体射流由于其在进料气体、添加剂和功率输入方面的灵活性，非常适合为等离子体驱动的生物催化原位产生HO，以适应具有高HO周转率的酶。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7b0/12409826/87d001533434/OPEN-14-e202500057-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7b0/12409826/efdc731d233f/OPEN-14-e202500057-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7b0/12409826/4b5f4bb21072/OPEN-14-e202500057-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7b0/12409826/9b88e3fbb543/OPEN-14-e202500057-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7b0/12409826/08ec6cda7cb6/OPEN-14-e202500057-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7b0/12409826/b4ece7dbfa8e/OPEN-14-e202500057-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7b0/12409826/21bbe1ccb3bf/OPEN-14-e202500057-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7b0/12409826/4f92fcf8a006/OPEN-14-e202500057-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7b0/12409826/87d001533434/OPEN-14-e202500057-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7b0/12409826/efdc731d233f/OPEN-14-e202500057-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7b0/12409826/4b5f4bb21072/OPEN-14-e202500057-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7b0/12409826/9b88e3fbb543/OPEN-14-e202500057-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7b0/12409826/08ec6cda7cb6/OPEN-14-e202500057-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7b0/12409826/b4ece7dbfa8e/OPEN-14-e202500057-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7b0/12409826/21bbe1ccb3bf/OPEN-14-e202500057-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7b0/12409826/4f92fcf8a006/OPEN-14-e202500057-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7b0/12409826/87d001533434/OPEN-14-e202500057-g008.jpg

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