Pradhan Dipransu, Das Mihir Tanay
P.G. Dept. of Environmental Science, Fakir Mohan University, Balasore, 756089, India.
Centre of Excellence for Bio-resource Management and Energy Conservation Material Development, Fakir Mohan University, Balasore, 756089, India.
Braz J Microbiol. 2025 Jun 23. doi: 10.1007/s42770-025-01710-0.
Terrestrial actinomycetes have historically dominated the production of antimicrobial compounds. However, recent investigations into aquatic strains, particularly those from marine habitats, have revealed a vast reservoir of bioactive compounds with potential therapeutic applications. This study focuses on actinomycetes isolated from Bichitrapur Mangrove sediments which creates an extreme habitat for the microorganisms. Six distinct colonies were isolated, and their potential for antimicrobial compound production was explored. The investigation integrates molecular and chemical analyses, employing genetic sequencing for taxonomic identification and gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS/MS) for compound characterization. The 16S rRNA gene sequences of BSS-D-04 and BSS-D-05 were submitted to the NCBI database, confirming their identities as Micromonospora sp. and Streptomyces sp., respectively. Phylogenetic analysis provided insight into the evolutionary relationships of these isolates. The organic solvent extracts from the selected strains were subjected to GC-MS and LC-MS/MS analyses, revealing many novel bioactive compounds. The identified compounds, documented for their broad-spectrum biological activities, encompassed antimicrobial, antifungal, insecticidal, antioxidative, anti-inflammatory, anticancer, antiviral, and antiparasitic properties. Especially, the study highlighted the higher pharmaceutical potential of Micromonospora sp. compared to Streptomyces sp. which is responsible for the 70% of the total discovered antibiotics. Hence, this comprehensive investigation sets the stage for further exploration and purification of metabolites from these highly potent actinobacterial strains which will help in new drug development and potential applications in the pharmaceutical industry.
陆生放线菌在历史上一直主导着抗菌化合物的生产。然而，最近对水生菌株，特别是来自海洋栖息地的菌株的研究发现了大量具有潜在治疗应用的生物活性化合物。本研究聚焦于从比奇特拉普尔红树林沉积物中分离出的放线菌，该沉积物为微生物创造了极端的栖息地。分离出六个不同的菌落，并探索了它们产生抗菌化合物的潜力。该研究整合了分子和化学分析，采用基因测序进行分类鉴定，并用气相色谱 - 质谱联用仪（GC-MS）和液相色谱 - 串联质谱仪（LC-MS/MS）对化合物进行表征。BSS-D-04和BSS-D-05的16S rRNA基因序列已提交至NCBI数据库，分别确认它们为小单孢菌属（Micromonospora sp.）和链霉菌属（Streptomyces sp.）。系统发育分析揭示了这些分离株的进化关系。对所选菌株的有机溶剂提取物进行GC-MS和LC-MS/MS分析，发现了许多新型生物活性化合物。所鉴定的化合物具有广泛的生物活性，包括抗菌、抗真菌、杀虫、抗氧化、抗炎、抗癌、抗病毒和抗寄生虫特性。特别是，该研究强调了小单孢菌属相较于链霉菌属具有更高的药用潜力，链霉菌属产生了已发现抗生素总量的70%。因此，这项全面的研究为进一步探索和纯化这些高效放线菌菌株的代谢产物奠定了基础，这将有助于新药开发及在制药行业的潜在应用。
