Leadbeater Daniel R, Setchfield Alexander T, Dowle Adam A, Bruce Neil C
Centre for Novel Agricultural Products, Department of Biology, University of York, Heslington, York YO10 5DD, United Kingdom.
Centre for Novel Agricultural Products, Department of Biology, University of York, Heslington, York YO10 5DD, United Kingdom.
Bioresour Technol. 2025 Nov;435:132864. doi: 10.1016/j.biortech.2025.132864. Epub 2025 Jun 21.
Lignocellulolytic enzyme production typically relies on submerged fungal fermentation (SmF) due to high operational control. However, fungi naturally thrive in environments with higher moisture and solid loadings representative of solid-state fermentation (SSF). This study investigates the secretome of Parascedosporium putredinis NO1 grown in SSF (35% w/v) and SmF (1.5% w/v) on wheat straw using a time-series exo-proteomics approach. Peak enzyme activity between conditions was comparable, however protein production in SSF was six-fold higher. SSF secretomes exhibited lower diversity of CAZYme families but a greater number of total upregulated proteins that included higher fold changes in cellulase CAZYme families such as GH1, GH3, GH6, and GH7 than SmF. Greater volatility in protein expression was observed in SmF, while SSF exhibited temporal stability. Here, it is shown that P. putredinis NO1 secretome is modified with significant changes in production, composition, diversity, and activity as a function of SFF when compared to SmF. These findings highlight SSF as a strategy to enhance fungal enzyme yield for lignocellulosic biomass valorization.
由于具有高度的操作可控性，木质纤维素酶的生产通常依赖于深层真菌发酵（SmF）。然而，真菌在水分含量较高且固体负载量较大的固态发酵（SSF）环境中能够自然生长。本研究采用时间序列外蛋白质组学方法，研究了在小麦秸秆上进行固态发酵（35% w/v）和深层发酵（1.5% w/v）培养的腐败拟青霉NO1的分泌组。两种培养条件下的酶活性峰值相当，但固态发酵中的蛋白质产量高出六倍。固态发酵分泌组中碳水化合物活性酶（CAZYme）家族的多样性较低，但上调的蛋白质总数较多，其中纤维素酶CAZYme家族（如GH1、GH3、GH6和GH7）的倍数变化高于深层发酵。深层发酵中观察到蛋白质表达的波动性更大，而固态发酵表现出时间稳定性。结果表明，与深层发酵相比，腐败拟青霉NO1的分泌组在产量、组成、多样性和活性方面随着固态发酵而发生显著变化。这些发现突出了固态发酵作为一种提高木质纤维素生物质增值真菌酶产量的策略。
