Naclerio R M, Plaut M, Adkinson N F, Norman P S
Int Arch Allergy Appl Immunol. 1985;78(3):225-32. doi: 10.1159/000233890.
To determine the effects of seasonal ragweed exposure on in vitro IgE antibody production, the antiragweed IgE antibody content of 7-day cultures of peripheral blood mononuclear cells was assessed in samples obtained before, during and after the ragweed season, in 4 ragweed-allergic and 11 nonallergic individuals. The levels of ragweed-specific IgE and total IgE in unstimulated culture supernatants were measured by solid phase radioimmunoassays. Antiragweed IgE antibody was apparently detectable in the 7-day culture supernatants of both allergic and nonallergic individuals, and the antibody concentrations increased after natural ragweed exposure. However, the bindable counts of IgE antibody in supernatants from allergic patients were inhibited by soluble ragweed antigen, while those from nonallergics were not inhibitable, suggesting that supernatants from nonallergics did not contain true ragweed-specific IgE antibody. Because, for most subjects, the quantity of IgE in culture supernatants from 7-day cultures of living cells was the same as that derived from cells which were either frozen and thawed prior to culture, or briefly exposed to pH 3.7 buffer, the majority of IgE antibody in supernatants from allergic subjects, and of total IgE in supernatants from all subjects, was apparently not newly synthesized, but was preformed, and appeared to be largely derived from the cell surface. IgE antiragweed antibody was detected in the nasal secretions and plasma of allergic, but not nonallergic individuals, and the levels of antibody in allergic subjects increased after seasonal exposure. The fraction of total IgE protein accounted for by IgE antiragweed antibody in nasal secretions collected postseasonally was higher than that in both culture supernatants and plasma, suggesting that ragweed-specific IgE antibody is synthesized locally in the nose.
为了确定季节性豚草暴露对体外IgE抗体产生的影响，在豚草季节之前、期间和之后采集的样本中，对4名豚草过敏个体和11名非过敏个体外周血单核细胞7天培养物中的抗豚草IgE抗体含量进行了评估。通过固相放射免疫测定法测量未刺激培养上清液中豚草特异性IgE和总IgE的水平。在过敏和非过敏个体的7天培养上清液中均明显可检测到抗豚草IgE抗体，并且在自然接触豚草后抗体浓度增加。然而，过敏患者上清液中IgE抗体的可结合计数受到可溶性豚草抗原的抑制，而非过敏患者的则不受抑制，这表明非过敏患者的上清液中不含有真正的豚草特异性IgE抗体。因为对于大多数受试者来说，活细胞7天培养上清液中的IgE量与培养前冷冻和解冻或短暂暴露于pH 3.7缓冲液的细胞中的IgE量相同，所以过敏受试者上清液中的大多数IgE抗体以及所有受试者上清液中的总IgE显然不是新合成的，而是预先形成的，并且似乎主要来源于细胞表面。在过敏个体而非非过敏个体的鼻分泌物和血浆中检测到IgE抗豚草抗体，并且过敏受试者的抗体水平在季节性暴露后增加。季节性接触豚草后收集的鼻分泌物中，IgE抗豚草抗体占总IgE蛋白的比例高于培养上清液和血浆中的比例，这表明豚草特异性IgE抗体是在鼻腔局部合成的。
