基于纵向核磁共振的男性山地超级马拉松运动员代谢组学分析：运动员监测与损伤预防的新视角
Longitudinal NMR-based Metabolomics Analysis of Male Mountain Ultramarathon Runners: New Perspectives for Athletes Monitoring and Injury Prevention.
作者信息
Schoumacher Matthieu, Nguyen Julie, Brevers Eric, Cirillo Arianna, Campas Manon, Grifnée Elodie, Demeuse Justine, Huyghebaert Loreen, Massonnet Philippe, Dubrowski Thomas, Ledeck Joy, Peeters Stéphanie, Millet Grégoire P, Croisille Pierre, Kaux Jean-François, de Tullio Pascal, Cavalier Etienne, Le Goff Caroline
机构信息
Clinical Metabolomics Group (CliMe), Center for Interdisciplinary Research On Medicines (CIRM), University of Liège, Liège, Belgium.
Department of Clinical Chemistry, University and University Hospital of Liège, Liège, Belgium.
出版信息
Sports Med Open. 2025 Jun 24;11(1):79. doi: 10.1186/s40798-025-00879-w.
BACKGROUND
The aim of this study was to explore how a metabolomic approach could provide valuable information on changes in the athletes' metabolome during a mountain ultramarathon race. To achieve this goal, we established a longitudinal cohort of athletes enrolled in the TOR des Géants, a 330 km mountain ultramarathon with 24,000 m of elevation gain. Sixteen healthy male athletes (43.9 ± 10.1 years) were recruited, and blood samples were collected at four time points: pre-race, mid-race, post-race and after 72 h recovery. Using a 1H-NMR-based metabolomic approach, we evaluated metabolic changes that occur during both race effort and recovery, and correlated them with functional muscle, cardiac, inflammatory, and renal biomarkers already used in the clinic. The processed data were analyzed using multivariate analysis tools specific to longitudinal study design, and innovative pathway analysis was used for data interpretation.
RESULTS
Mountain ultramarathon running significantly affected the metabolism and physiology of athletes. Multivariate analysis highlighted specific metabolites and functional biomarkers associated with prolonged exercise. Neither metabolite levels nor biomarker concentrations returned to baseline after 3 days of recovery. Finally, innovative pathway analysis shed light on specific metabolic changes resulting from mountain ultramarathon exercise.
CONCLUSION
In this study, we propose an NMR-based metabolomics strategy to assess exercise-associated metabolic changes during and after events such as the Tor des Géants. Using state-of-the-art data representation methods specific to metabolomics analysis, we demonstrated that such a methodology can provide a unique view of the biology associated with such extreme conditions. As this approach provides unique insights into the biology of extreme exercise, it holds promise for the development of new tools for athlete management.
背景
本研究的目的是探索代谢组学方法如何能够提供有关运动员在山地超级马拉松比赛期间代谢组变化的有价值信息。为实现这一目标，我们建立了一个参加巨人之旅（TOR des Géants）的运动员纵向队列，这是一场330公里的山地超级马拉松比赛，海拔上升24000米。招募了16名健康男性运动员（43.9±10.1岁），并在四个时间点采集血样：赛前、赛中、赛后以及恢复72小时后。使用基于1H-NMR的代谢组学方法，我们评估了比赛过程和恢复期间发生的代谢变化，并将其与临床中已经使用的功能性肌肉、心脏、炎症和肾脏生物标志物相关联。使用特定于纵向研究设计的多变量分析工具对处理后的数据进行分析，并使用创新的通路分析进行数据解释。
结果
山地超级马拉松跑步对运动员的代谢和生理产生了显著影响。多变量分析突出了与长时间运动相关的特定代谢物和功能性生物标志物。恢复3天后，代谢物水平和生物标志物浓度均未恢复到基线。最后，创新的通路分析揭示了山地超级马拉松运动导致的特定代谢变化。
结论
在本研究中，我们提出了一种基于NMR的代谢组学策略，以评估诸如巨人之旅等赛事期间和之后与运动相关的代谢变化。使用特定于代谢组学分析的最新数据表示方法，我们证明了这种方法可以提供与这种极端条件相关的生物学的独特视角。由于这种方法为极端运动生物学提供了独特的见解，因此有望开发用于运动员管理的新工具。
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