Zhao Yong, Wang Chunyuan, Wu Yan, Xiang Jin, Zhang Yiyu
Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, West Campus, Guiyang, Guizhou, People's Republic of China.
Institute of Xiang Pigs, Guizhou University, West Campus, Guiyang, Guizhou, People's Republic of China.
PLoS One. 2025 Jul 14;20(7):e0311064. doi: 10.1371/journal.pone.0311064. eCollection 2025.
This study aimed to investigate the effects of single nucleotide polymorphisms (SNPs) in progestogen-associated endometrial protein (PAEP), keratin 10 (KRT10), and bone morphogenetic protein 7 (BMP7) genes on reproductive traits (total number of piglets born, number of piglets born alive, litter birth weight, number of piglets weaned and litter weight weaned) in Kele pigs. We used 255 multiparous Kele sow (2-4 parities) as research materials. SNPs were identified by using a PCR amplification instrument and sequence alignment software DANMAN. The population genetic characteristics of SNPs were analyzed using the SHEsis online software. Bioinformatics analyses of SNPs were conducted using RNAfold, SOPMA, SWISS-MODEL, and Swiss-PdbViewer programs. The associations between the SNPs and reproductive traits in Kele pigs were analyzed through SPSS 22.0 software. In this study, nine SNPs were identified in the three genes: g.1884992 T > C (exon 4), g.1885125 G > C (intron 4), and g.1885158 G > A (intron 4) in PAEP, g.21643703 C > T (intron 4), g.21643714 G > A (intron 4) and g.21643741 G > A (exon 5) in KRT10, and g.57647887 G > A (intron 3), g.57647990 C > T (intron 3) and g.57648145 C > G (intron 3) in BMP7. In SNPs g.1884992 T > C of PAEP, missense mutation eventuated structural changes in mRNA and proteins' secondary structure. In SNPs g.21643741 G > A of KRT10, the synonymous mutation led to an alteration in mRNA secondary structure. For PAEP, the CC genotype in SNPs g.1885125 G > C and the AA genotype in SNPs g.1885158 G > A showed significantly higher values than other genotypes in all reproduction traits except for litter birth weight, preliminarily identified as the favorable genotypes. For KRT10, the GG genotype in SNPs g.21648641 G > A showed significant superiority to the AA genotype (P < 0.05) in all aspects except for litter birth weight and notably surpassed the GA genotype in the total number of piglets born (P < 0.05), preliminarily recognized as a favorable genotype. Regarding BMP7, the GA genotype in SNPs g.57647887 G > A and the CT genotype in SNPs g.57647990 C > T exhibited significantly higher number of piglets born alive and number of piglets born alive compared to other genotypes (P < 0.05). The GG genotype in SNPs g.57648145 C > G was significantly associated with higher litter birth weight (P < 0.05). The result of diplotype analyses indicated that the H3H3 (CCGGGG) of PAEP and H3H3 (CCGGAA) of KRT10 had a significant effect on the five traits. For BMP7, the H4H4 (AATTGG) diplotype showed a significant influence on all genotypes except litter birth weight.
本研究旨在探讨孕激素相关子宫内膜蛋白（PAEP）、角蛋白10（KRT10）和骨形态发生蛋白7（BMP7）基因中的单核苷酸多态性（SNP）对柯乐猪繁殖性状（产仔总数、产活仔数、窝出生重、断奶仔猪数和断奶窝重）的影响。我们以255头经产柯乐母猪（2-4胎）作为研究材料。使用PCR扩增仪和序列比对软件DANMAN鉴定SNP。利用SHEsis在线软件分析SNP的群体遗传特征。使用RNAfold、SOPMA、SWISS-MODEL和Swiss-PdbViewer程序对SNP进行生物信息学分析。通过SPSS 22.0软件分析柯乐猪中SNP与繁殖性状之间的关联。在本研究中，在这三个基因中鉴定出9个SNP：PAEP基因中的g.1884992 T＞C（外显子4）、g.1885125 G＞C（内含子4）和g.1885158 G＞A（内含子4），KRT10基因中的g.21643703 C＞T（内含子4）、g.21643714 G＞A（内含子4）和g.21643741 G＞A（外显子5），以及BMP7基因中的g.
