Uozumi T, Hoshino T, Miwa K, Horinouchi S, Beppu T, Arima K
Mol Gen Genet. 1977 Mar 28;152(1):65-9. doi: 10.1007/BF00264941.
Host specific restriction was detected in 13 Bacillus strains, when 63 strains of Bacillus subtilis and 15 other Bacillus strains were tested with phage phi 105C. These 13 strains were classified into 8 groups (M,H,C,N,E,F,G,P) by the type of restriction. M-type strains (B. subtilis Marburg 168, its derivatives, and two other strains) showed relatively weak restriction, restricting phi 105C from other groups of Bacillus by ratios of 10(-1) to 10(-3). Strains of groups H,C,N,E,F,G, and P restricted phi 105C from other groups by ratios of 10(-2) to 10(-8). It was confirmed with some of the strains that type-specific modification was endowed only by the last host. Furthermore, we isolated one restriction deficient mutant of B. subtilis Marburg 168-YS11, which had also lost its modification phenotype.
当用噬菌体phi 105C对63株枯草芽孢杆菌和15株其他芽孢杆菌菌株进行测试时，在13株芽孢杆菌菌株中检测到宿主特异性限制。根据限制类型，这13株菌株被分为8组（M、H、C、N、E、F、G、P）。M型菌株（枯草芽孢杆菌马尔堡168及其衍生物，以及另外两株菌株）表现出相对较弱的限制，以10^(-1)至10^(-3)的比例限制来自其他芽孢杆菌组的phi 105C。H、C、N、E、F、G和P组的菌株以10^(-2)至10^(-8)的比例限制来自其他组的phi 105C。用一些菌株证实，类型特异性修饰仅由最后一个宿主赋予。此外，我们分离出了枯草芽孢杆菌马尔堡168 - YS11的一个限制缺陷突变体，它也失去了其修饰表型。
