Zakharova M S, Shirko G N, Gureeva A A, Pereverzev N A
Zh Mikrobiol Epidemiol Immunobiol. 1976 Nov(11):103-8.
The authors present the results of studying the protective and sensitizing properties of a new preparation made of a ultrasonic disintegrate of pertussis microbes treated by ethyl ether. As shown by electron microscopy, the preparation consisted of the cell wall elements (the membrane), remnants of the cytoplasm and protectosome, i.e. it represented a vaccine consisting of cell fragments. In crude and sorbed condition it possessed marked protective properties (a test on mice). The content of protective units in the adsorbed preparation increased 1.5-3 times. The vaccine produced no sensitizing action, and its histamine-sensitizing activity was 3-5 times lower by protein and 5-10 times--by IOU than that of the whole-cell vaccine prepared form the same microbial suspension.
作者介绍了对一种由经乙醚处理的百日咳微生物超声裂解物制成的新制剂的保护和致敏特性的研究结果。电子显微镜显示，该制剂由细胞壁成分（细胞膜）、细胞质残余物和保护体组成，即它是一种由细胞碎片组成的疫苗。在粗制和吸附状态下，它具有显著的保护特性（对小鼠的试验）。吸附制剂中保护单位的含量增加了1.5至3倍。该疫苗无致敏作用，其组胺致敏活性按蛋白质计算比由相同微生物悬体制备的全细胞疫苗低3至5倍，按国际单位计算低5至10倍。
