Kara Hatibe, Turkmen Alemdar Nihal
Department of Biochemistry, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Bursa, Turkey.
Department of Biochemistry, Faculty of Veterinary Medicine, Balikesir University, 10100 Balikesir, Turkey.
Antibiotics (Basel). 2025 May 30;14(6):563. doi: 10.3390/antibiotics14060563.
Antibiotics are antimicrobial drugs used to treat and prevent infections. Unconscious use of antibiotics leads to many negative results. This study aimed to emphasize the negative aspects of antibiotics by determining their effects on purified enzymes. Beta glucosidase enzymes (BGLs) were purified from rat liver and kidney tissues using the sepharose-4B-LTyrosine-1-Naphthylamine hydrophobic interaction chromatography method. Liver BGL enzyme was purified 30.2-fold with a yield of 43.4%, while kidney BGL was purified 5.1-fold with a yield of 12.2%. Purified enzymes were visualized using SDS-PAGE. In vitro inhibition effects of ampicillin cefuroxime, amoxicillin-clavulanate, cefazolin sodium, gentamicin, and ceftriaxone antibiotics were determined on purified BGLs. Ampicillin was found to inhibit rat liver and kidney BGLs competitively and uncompetitively, with IC50 values of 69.56 and 25.30 mM, respectively. Other antibiotics investigated did not significantly reduce liver BGL activity. Cefuroxime inhibited rat kidney BGL uncompetitively with IC50 values of 76.88 mM, while amoxicillin-clavulanate and cefazolin sodium inhibited it noncompetitively, with IC50 values of 41.32 and 98.81 mM, respectively. Gentamicin and ceftriaxone, whose effects were investigated, did not reduce kidney BGL activity. Some of the commonly used antibiotics reduce liver and kidney BGL activity, and this indicates that they may potentially impair metabolic functions. These results emphasize that caution should be exercised when using antibiotics.
抗生素是用于治疗和预防感染的抗菌药物。抗生素的不合理使用会导致许多负面结果。本研究旨在通过测定抗生素对纯化酶的影响来强调其负面作用。采用琼脂糖-4B-L-酪氨酸-1-萘胺疏水相互作用色谱法从大鼠肝脏和肾脏组织中纯化β-葡萄糖苷酶（BGLs）。肝脏BGL酶纯化了30.2倍，产率为43.4%，而肾脏BGL酶纯化了5.1倍，产率为12.2%。使用SDS-PAGE对纯化的酶进行可视化分析。测定了氨苄西林、头孢呋辛、阿莫西林-克拉维酸、头孢唑林钠、庆大霉素和头孢曲松等抗生素对纯化BGLs的体外抑制作用。发现氨苄西林对大鼠肝脏和肾脏BGLs具有竞争性和非竞争性抑制作用，IC50值分别为69.56和25.30 mM。所研究的其他抗生素未显著降低肝脏BGL活性。头孢呋辛对大鼠肾脏BGL具有非竞争性抑制作用，IC50值为76.88 mM，而阿莫西林-克拉维酸和头孢唑林钠对其具有非竞争性抑制作用，则IC50值分别为41.32和98.81 mM。所研究的庆大霉素和头孢曲松未降低肾脏BGL活性。一些常用抗生素会降低肝脏和肾脏BGL活性，这表明它们可能会潜在损害代谢功能。这些结果强调在使用抗生素时应谨慎。
