Aguirre-Noyola José Luis, Ramírez-Mosqueda Marco A, Cadena-Zamudio Jorge David, Caamal-Velázquez José Humberto, Cruz-Gutiérrez Esmeralda J, Armenta-Medina Alma
Centro Nacional de Recursos Genéticos-INIFAP, Boulevard de la Biodiversidad 400, Rancho las Cruces 47600, Tepatitlán de Morelos, Jalisco, Mexico.
Colegio de Postgraduados Campus Campeche, Haltunchén-Edzná km 17.5, Sihochac 24450, Champotón, Campeche, Mexico.
BioTech (Basel). 2025 Jun 6;14(2):45. doi: 10.3390/biotech14020045.
Nanobiotechnology applications in plant tissue culture have improved the development and physiology of explants, resulting in plants with high genetic homogeneity and phytosanitary quality. Silver nanoparticles (AgNPs) are well-known for their microbicidal properties, but their biochemical effects on plants require further exploration. In this work, green-synthesized AgNPs were evaluated in strawberry in vitro culture, photosynthetic pigment production, and acclimatization. AgNPs produced by were characterized. Strawberry explants were grown in vitro on MS medium with 0, 100, 200, and 300 mg L AgNPs at 24 ± 2 °C and a photoperiod of 16:8 h light/dark. Shoot height and number, number of leaves, number of roots, and root length were evaluated, and chlorophyll (a, b, and total) was quantified. Rooted shoots were acclimatized ex vitro on substrates containing 0 and 200 mg L AgNPs. The results showed that low AgNPs concentrations had a positive impact on shoot multiplication, development, and rooting, but at higher concentrations, the effects decayed. However, chlorophyll production improved with increasing AgNP concentration. Shoots treated with AgNPs showed higher ex vitro survival. Our study has direct implications for the profitability and sustainability of commercial strawberry production.
纳米生物技术在植物组织培养中的应用改善了外植体的发育和生理状况，从而培育出具有高度遗传同质性和植物检疫质量的植株。银纳米颗粒（AgNPs）因其杀菌特性而闻名，但其对植物的生化影响仍需进一步探索。在这项研究中，对绿色合成的AgNPs在草莓离体培养、光合色素产生和驯化方面进行了评估。对由[此处缺失相关内容]产生的AgNPs进行了表征。草莓外植体在添加了0、100、200和300 mg/L AgNPs的MS培养基上于24±2°C、16:8小时光/暗的光周期条件下进行离体培养。评估了茎高、茎数、叶数、根数和根长，并对叶绿素（a、b和总量）进行了定量分析。将生根的茎在含有0和200 mg/L AgNPs的基质上进行离体驯化。结果表明，低浓度的AgNPs对茎的增殖、发育和生根有积极影响，但在较高浓度下，这种影响减弱。然而，叶绿素的产生随着AgNP浓度的增加而改善。用AgNPs处理的茎在离体条件下表现出更高的存活率。我们的研究对商业草莓生产的盈利能力和可持续性具有直接影响。
