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琼脂糖凝胶电泳揭示眼眶成纤维细胞产生的透明质酸的分子量分布。

Agarose Gel Electrophoresis Reveals the Molecular Weight Distribution of Hyaluronan Produced by Orbital Fibroblasts.

作者信息

Galgoczi Erika, Katko Monika, Borbely Sara, Orsos Istvan, Molnar Zsanett, Ujhelyi Bernadett, Steiber Zita, Nagy Endre V

机构信息

Division of Endocrinology, Department of Internal Medicine, Faculty of Medicine, University of Debrecen, Nagyerdei körút 98, 4032 Debrecen, Hungary.

Doctoral School of Health Sciences, University of Debrecen, 4032 Debrecen, Hungary.

出版信息

Gels. 2025 May 29;11(6):406. doi: 10.3390/gels11060406.

DOI:10.3390/gels11060406
PMID:40558705
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12191666/
Abstract

Thyroid eye disease (TED) is characterized by autoimmune inflammation and structural remodelling of orbital tissues, which is a consequence of the activation of orbital fibroblasts (OFs). As a result of this activation, the production of hyaluronan (HA) and the proliferation and adipocyte differentiation of OFs are enhanced. Adipogenesis leads to additional accumulation of HA. The aim of this study was to elucidate the molecular weight distribution of HA produced by OFs under basic conditions and after adipogenic stimuli. The concentration and the molecular weight distribution of HA were examined using ELISA and agarose gel electrophoresis, respectively, in TED (n = 3) and non-TED (n = 3) OF cultures. Under adipogenic stimuli, HA production is increased in OFs. In TED OF cultures, which, unlike non-TED OFs, can differentiate into adipocytes, the enhanced proportion of high-molecular-weight (HMW) HA of more than 2000 kDa is responsible for the increased HA concentration in the culture media. In non-TED OF cultures, which contain a negligible number of differentiating cells after adipogenic stimulation, the medium-molecular-weight (MMW) HA fragments from 50 to 1000 kDa also contribute to the enhanced HA content. Increased production of HMW-HA during adipocyte differentiation of TED OFs is responsible for the elevated HA content in the culture media, which may be an important contributor to both connective tissue matrix expansion and edema in the pathogenesis of TED.

摘要

甲状腺眼病（TED）的特征是眼眶组织的自身免疫性炎症和结构重塑，这是眼眶成纤维细胞（OFs）激活的结果。由于这种激活，透明质酸（HA）的产生以及OFs的增殖和脂肪细胞分化增强。脂肪生成导致HA进一步积累。本研究的目的是阐明在基础条件下和成脂刺激后OFs产生的HA的分子量分布。分别使用酶联免疫吸附测定（ELISA）和琼脂糖凝胶电泳检测TED（n = 3）和非TED（n = 3）的OF培养物中HA的浓度和分子量分布。在成脂刺激下，OFs中HA的产生增加。在TED的OF培养物中，与非TED的OFs不同，其可以分化为脂肪细胞，大于2000 kDa的高分子量（HMW）HA比例增加是培养基中HA浓度增加的原因。在非TED的OF培养物中，成脂刺激后含有可忽略不计数量的分化细胞，50至1000 kDa的中分子量（MMW）HA片段也导致HA含量增加。TED的OFs在脂肪细胞分化过程中HMW-HA产生增加是培养基中HA含量升高的原因，这可能是TED发病机制中结缔组织基质扩张和水肿的重要促成因素。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d81/12191666/6412a6482751/gels-11-00406-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d81/12191666/1dcbc78492a3/gels-11-00406-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d81/12191666/c60fa2ce19c3/gels-11-00406-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d81/12191666/0c75153fa43c/gels-11-00406-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d81/12191666/6412a6482751/gels-11-00406-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d81/12191666/1dcbc78492a3/gels-11-00406-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d81/12191666/3ec7f5035be9/gels-11-00406-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d81/12191666/71f5acba3ea5/gels-11-00406-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d81/12191666/ea31d747dfac/gels-11-00406-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d81/12191666/88052dfe308f/gels-11-00406-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d81/12191666/dcf69d239f14/gels-11-00406-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d81/12191666/6e7486fa012a/gels-11-00406-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d81/12191666/c60fa2ce19c3/gels-11-00406-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d81/12191666/0c75153fa43c/gels-11-00406-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d81/12191666/6412a6482751/gels-11-00406-g010.jpg

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