Vladkova Todorka G, Gospodinova Dilyana N, Dineff Peter D, Keremidarska-Markova Milena, Hristova-Panusheva Kamelia, Krasteva Natalia
Department of Polymer Engineering, Faculty of Chemical Technologies, University of Chemical Technology and Metallurgy, 1756 Sofia, Bulgaria.
Department of Electrical Apparatus, Electro-Technical Faculty, Technical University of Sofia, 1797 Sofia, Bulgaria.
J Funct Biomater. 2025 Jun 12;16(6):219. doi: 10.3390/jfb16060219.
Electrospun collagen-based fibrous mats are of increasing interest for cell culture, regenerative medicine, and tissue engineering. The focus of this investigation is on the assessment of the electrospinning ability of bovine split hide collagen (BSHC), the effect of glycosaminoglycan (GAG) incorporation on the mats' structural morphology, and the impact on the adhesion and proliferation of human adipose-derived mesenchymal stem cells (hAD-MSCs). Electrospun mats were prepared using benign and fluoroalcohol solutions of BSHC and BSHC/GAGs under varied operation conditions. SEM observations and analysis were employed to characterize the structural morphology of the mats. Several parameters were used to evaluate the hAD-MSC behavior: cytotoxicity, cell morphology, cell number and spreading area, cytoskeleton, focal adhesion contacts, and cell proliferation. Electrospinning using benign solvents was impossible. However, fiber mats were successfully prepared from hexafluoropropanol (HFP) solutions. Different structural morphologies and fiber diameters of the electrospun mats were observed depending on the composition and concentration of the electrospinning solutions. Both BSHC and BSHC/GAG mats supported the in vitro adhesion, growth, and differentiation of hAD-MSCs, with some variations based on their composition and structural morphology. The absence of cytotoxicity and the good hAD-MSC adhesiveness make them promising substrates for cell adhesion, proliferation, and further stem cell differentiation.
基于电纺胶原蛋白的纤维垫在细胞培养、再生医学和组织工程领域越来越受到关注。本研究的重点是评估牛皮分层胶原（BSHC）的电纺能力、糖胺聚糖（GAG）掺入对垫子结构形态的影响，以及对人脂肪间充质干细胞（hAD-MSCs）黏附和增殖的影响。在不同的操作条件下，使用BSHC和BSHC/GAGs的良性和氟醇溶液制备电纺垫。采用扫描电子显微镜（SEM）观察和分析来表征垫子的结构形态。使用了几个参数来评估hAD-MSC的行为：细胞毒性、细胞形态、细胞数量和铺展面积、细胞骨架、粘着斑接触和细胞增殖。使用良性溶剂进行电纺是不可能的。然而，成功地从六氟异丙醇（HFP）溶液中制备了纤维垫。根据电纺溶液的组成和浓度，观察到电纺垫具有不同的结构形态和纤维直径。BSHC和BSHC/GAG垫子均支持hAD-MSCs的体外黏附、生长和分化，根据其组成和结构形态存在一些差异。无细胞毒性以及良好的hAD-MSC黏附性使其成为细胞黏附、增殖和进一步干细胞分化的有前景的底物。
