Zhao Kuan, Mao Mingwei, Wang Xianghua, Xu Jianping
Key Laboratory of Natural Microbial Medicine Research of Jiangxi Province, College of Life Science, Jiangxi Science and Technology Normal University, Nanchang 330013, China.
Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
J Fungi (Basel). 2025 Jun 8;11(6):438. doi: 10.3390/jof11060438.
is an economically important wild edible fungus in sub-tropical and temperate Asia. At present, little is known about its genetic diversity, mode of reproduction, and population structure in natural environments. In this study, we collected 102 specimens from eight geographic locations across three provinces in southwest China. Five simple sequence repeat markers that demonstrated high polymorphism were developed and used to analyze the patterns of genetic variations within and among the eight geographic populations. Analyses of molecular variance revealed that 60% of the observed genetic variation occurred among populations, with the remaining 40% attributable to within populations, while inter-provincial variation was nonsignificant. Combined analyses based on discriminant analysis of principal components, STRUCTURE, and the unweighted pair group method with arithmetic mean identified two distinct genetic subpopulations with each genetic subpopulation showing a wide geographical distribution, a result consistent with ancient divergence and recent gene flow within this species in southwest China. Interestingly, excess homozygosity was observed at most loci in almost all of the eight geographic populations, consistent with inbreeding being common for this species in nature. Together, our results revealed the genetic diversity, mode of reproduction, and geographic structuring of this important wild mushroom in southwest China.
是亚洲亚热带和温带地区一种具有重要经济价值的野生可食用真菌。目前，对于其自然环境中的遗传多样性、繁殖方式和种群结构知之甚少。在本研究中，我们从中国西南部三个省份的八个地理位置收集了102个标本。开发了五个显示出高多态性的简单序列重复标记，并用于分析八个地理种群内部和之间的遗传变异模式。分子方差分析表明，观察到的遗传变异中有60%发生在种群之间，其余40%归因于种群内部，而省际变异不显著。基于主成分判别分析、STRUCTURE和算术平均的非加权配对组方法的综合分析确定了两个不同的遗传亚群，每个遗传亚群都显示出广泛的地理分布，这一结果与中国西南部该物种的古代分化和近期基因流动一致。有趣的是，在几乎所有八个地理种群的大多数位点都观察到了纯合子过剩，这与该物种在自然界中常见近亲繁殖一致。总之，我们的结果揭示了中国西南部这种重要野生蘑菇的遗传多样性、繁殖方式和地理结构。
