Bonthong Pawarin, Bunterngsook Benjarat, Mhuantong Wuttichai, Aiewviriyasakul Katesuda, Sritusnee Wipawee, Champreda Verawat, Lekakarn Hataikarn
Department of Biotechnology, Faculty of Science and Technology, Rangsit Campus, Thammasat University, Pathum Thani 12120, Thailand.
Enzyme Technology Research Team, Biorefinery Technology and Bioproduct Research Group, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Khlong Luang, Pathum Thani 12120, Thailand.
J Fungi (Basel). 2025 Jun 13;11(6):453. doi: 10.3390/jof11060453.
The development of yeast cell factories for efficient xylose utilization and xylitol production is crucial for advancing sustainable biotechnological processes. Xylose, a major component of lignocellulosic biomass, presents challenges for microbial conversion due to its complex metabolic pathways. This study presents the genomic perspective and xylitol production capability of a novel xylose utilizing yeast TBRC 4498. Genome sequencing and functional annotation revealed key metabolic networks and genes involved in the xylose metabolism pathway, providing insights into the strain's performance. The TBRC 4498 had excellent growth and xylose assimilation at a broad range of xylose concentrations from 40 to 140 g/L, with the highest growth rate at 80 g/L of xylose. The highest xylitol production yield (83.19 g/L) was detected from 120 g/L of xylose at 30 °C for 72 h, equivalent to 0.65 g xylitol/g xylose and 1.16 g/L/h productivity. Remarkably, TBRC 4498 produced high-purity xylitol, achieving over 95% homogeneity without forming undesirable byproducts, such as acid or ethanol. These results demonstrated the potential of TBRC 4498 as a whole-cell biocatalyst for xylitol production using high xylose concentrations, offering a promising microbial cell factory for large-scale xylitol production from lignocellulosic sugar.
开发能够高效利用木糖并生产木糖醇的酵母细胞工厂对于推进可持续生物技术工艺至关重要。木糖是木质纤维素生物质的主要成分，由于其复杂的代谢途径，给微生物转化带来了挑战。本研究展示了一种新型木糖利用酵母TBRC 4498的基因组视角和木糖醇生产能力。基因组测序和功能注释揭示了木糖代谢途径中涉及的关键代谢网络和基因，为该菌株的性能提供了见解。TBRC 4498在40至140 g/L的广泛木糖浓度范围内具有出色的生长和木糖同化能力，在80 g/L木糖时生长速率最高。在30°C下培养72小时，从120 g/L木糖中检测到最高木糖醇产量（83.19 g/L），相当于0.65 g木糖醇/g木糖和1.16 g/L/h的生产率。值得注意的是，TBRC 4498生产出高纯度木糖醇，纯度超过95%，且未形成酸或乙醇等不良副产物。这些结果证明了TBRC 4498作为使用高浓度木糖生产木糖醇的全细胞生物催化剂的潜力，为从木质纤维素糖大规模生产木糖醇提供了一个有前景的微生物细胞工厂。
