Furuya Runa, Takei Satomi, Tabe Yoko, Ablordey Anthony, Saito Ryoichi
Department of Molecular Microbiology and Immunology, Graduate School of Medicine and Dental Science, Institute of Science Tokyo, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
Pathogens. 2025 May 23;14(6):522. doi: 10.3390/pathogens14060522.
, a gram-negative, non-lactose-fermenting, aerobic, rod-shaped bacterium, is found in environmental sources (e.g., soil and water). In 2020, was reclassified as . We conducted a genomic analysis of from hospital wastewater samples in western Ghana. A hybrid genome assembly was constructed integrating short-read data from DNA Nanoball sequencing with long-read sequences generated by Oxford Nanopore MinION technology. Identification and antimicrobial susceptibility profiles were determined using MicroScan autoSCAN-4 based on Clinical and Laboratory Standard Institute documents. ResFinder and CARD Resistance Gene Identifier (RGI) were used to identify antimicrobial resistance (AMR) genes, and BLAST and VFDB datasets were used to identify virulence factor genes. The complete genome had two chromosomes, no plasmid, and a high average nucleotide identity value (98.05%) with . Resistance to trimethoprim-sulfamethoxazole was revealed, the first report in this species. CARD RGI revealed the presence of AMR genes, including ANT(9)-Ic and . Local BLAST analysis revealed Cgs, a virulence factor. is an opportunistic human pathogen clinically isolated several times, suggesting the importance of accurately identifying multidrug resistance. may possess virulence factors similar to those of . Further study is needed to fully elucidate its pathogenesis.
[细菌名称]是一种革兰氏阴性、不发酵乳糖、需氧的杆状细菌，存在于环境来源（如土壤和水）中。2020年，[细菌名称]被重新分类为[新的细菌名称]。我们对加纳西部医院废水样本中的[细菌名称]进行了基因组分析。构建了一个混合基因组组装体，将DNA纳米球测序的短读数据与牛津纳米孔MinION技术生成的长读序列整合在一起。根据临床和实验室标准协会的文件，使用MicroScan autoSCAN-4确定鉴定和抗菌药物敏感性谱。使用ResFinder和CARD耐药基因标识符（RGI）来鉴定抗菌药物耐药（AMR）基因，使用BLAST和VFDB数据集来鉴定毒力因子基因。完整基因组有两条染色体，无质粒，与[参考细菌名称]的平均核苷酸同一性值较高（98.05%）。发现对甲氧苄啶-磺胺甲恶唑耐药，这是该物种的首次报道。CARD RGI显示存在AMR基因，包括ANT(9)-Ic和[具体基因名称]。局部BLAST分析显示存在Cgs，一种[细菌名称]毒力因子。[细菌名称]是一种临床上多次分离出的机会性人类病原体，表明准确识别多重耐药性的重要性。[细菌名称]可能具有与[参考细菌名称]相似的毒力因子。需要进一步研究以充分阐明其发病机制。
