Ferraro Laetitia, Briolant Sébastien, Nacher Mathieu, Vezenegho Samuel, Adde Antoine, Nguyen Christophe, Gaborit Pascal, Issaly Jean, Carinci Romuald, Pommier de Santi Vincent, Girod Romain, Dusfour Isabelle, Bogreau Hervé
Unité d'Entomologie Médicale, Institut Pasteur de la Guyane, 97306 Cayenne, France.
Unité de Parasitologie et Entomologie, Département de Microbiologie et Maladies Infectieuses, Institut de Recherche Biomédicale des Armées (IRBA), 13005 Marseille, France.
Trop Med Infect Dis. 2025 Jun 18;10(6):174. doi: 10.3390/tropicalmed10060174.
is a major malaria vector in South America. Understanding its population dynamics is critical for designing effective vector control strategies. While various collection methods exist, they may sample distinct populations. Microsatellite genotyping across nine loci was performed to characterize populations, which were collected in French Guiana between 6:30 p.m. and 7:00 a.m. using human landing catch (HLC) or Mosquito Magnet (MM) traps. Traps were arranged in a 3 × 3 Latin square design to minimize possible effects of geographical position. Pairwise index and discriminant analyses of principal components (DAPC) were used to make comparisons. A total of 431 were analyzed. No significant genetic differentiation was observed between collection methods or time slots ( values non-significant, > 0.25), with DAPC revealing a single genetic cluster. Despite documented phenotypic variations, no significant population structure was detected among sampled in a rural village in French Guiana via collection methods or time slots. These findings confirm that mosquitoes collected with these various methods or time slots are suitable for the molecular studies of in French Guiana. In this context, Mosquito Magnet traps could also represent an alternative to the now controversial human landing catch.
是南美洲主要的疟疾传播媒介。了解其种群动态对于设计有效的媒介控制策略至关重要。虽然存在各种采集方法，但它们可能采集到不同的种群。对9个位点进行微卫星基因分型以表征种群，这些种群于下午6:30至上午7:00在法属圭亚那使用人饵诱捕法（HLC）或诱蚊灯（MM）诱捕器采集。诱捕器按3×3拉丁方设计排列，以尽量减少地理位置可能产生的影响。使用成对指数和主成分判别分析（DAPC）进行比较。共分析了431只。在采集方法或时间段之间未观察到显著的遗传分化（值不显著，>0.25），DAPC显示为单一遗传簇。尽管有记录的表型变异，但通过采集方法或时间段在法属圭亚那一个乡村采集的样本中未检测到显著的种群结构。这些发现证实，用这些不同方法或时间段采集的蚊子适用于法属圭亚那的分子研究。在这种情况下，诱蚊灯诱捕器也可能是现在存在争议的人饵诱捕法的一种替代方法。
