Vadney F S, Hawkins J E
J Clin Microbiol. 1985 Nov;22(5):884-5. doi: 10.1128/jcm.22.5.884-885.1985.
Surface paper strips containing hemin (X factor) were tested on Middlebrook 7H10 agar and Lowenstein-Jensen medium to determine if the growth requirement of Mycobacterium haemophilum for iron-containing complexes could be met by this simplified method. One reference strain and seven strains of this species isolated from patients showed good growth around the strips on 7H10 within 2 weeks but failed to grow on Lowenstein-Jensen medium with X-factor strips. Middlebrook 7H10 medium with X-factor strips may be a useful alternative to the preparation of specialized media for the recovery of M. haemophilum from clinical specimens.
将含有血红素（X因子）的表面纸条在Middlebrook 7H10琼脂和罗氏培养基上进行测试，以确定这种简化方法是否能够满足嗜血性分枝杆菌对含铁复合物的生长需求。从患者中分离出的1株参考菌株和7株该菌种在7H10培养基上，纸条周围2周内生长良好，但在添加X因子纸条的罗氏培养基上未能生长。含X因子纸条的Middlebrook 7H10培养基可能是从临床标本中分离嗜血性分枝杆菌时，制备专用培养基的一种有用替代方法。
