• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

高溶解性贻贝足蛋白通过PI3K/Akt和Nrf2/HO-1信号通路增强抗氧化防御和细胞保护作用。

Highly Soluble Mussel Foot Protein Enhances Antioxidant Defense and Cytoprotection via PI3K/Akt and Nrf2/HO-1 Pathways.

作者信息

Li Na, Xu Jiren, Liu Boheng, Elango Jeevithan, Wu Wenhui

机构信息

Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.

Department of Biomaterials Engineering, Faculty of Health Sciences, UCAM-Universidad Católica San Antonio de Murcia, Guadalupe, 30107 Murcia, Spain.

出版信息

Antioxidants (Basel). 2025 May 27;14(6):644. doi: 10.3390/antiox14060644.

DOI:10.3390/antiox14060644
PMID:40563279
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12189154/
Abstract

Mussel foot protein is a bioadhesive protein with potential biomedical applications, but its limited solubility and poor biological stability hinder its widespread use. In this study, highly soluble mussel foot protein (HMFP) was successfully extracted using a stepwise selective enzymatic digestion method, with a molecular weight in the range of 11-17 kDa. Furthermore, a dual-functional polyethylene glycol (PEG) derivative of HMFP, designated HMFP-PEG, was synthesized. FTIR analysis confirmed the successful modification of HMFP with PEG, while TGA analysis and SEM observations demonstrated that PEG modification significantly enhanced the stability of the protein. Both HMFP and HMFP-PEG effectively scavenged free radicals, enhanced antioxidant enzyme activity, and reduced MDA levels. Additionally, they activated the PI3K/Akt and Nrf2/HO-1 signaling pathways, inhibiting HO-induced cell apoptosis. Notably, HMFP-PEG exhibited superior antioxidant and cell-protective effects compared to HMFP, suggesting that PEG modification improves the functional stability of the protein. This study provides theoretical support for the potential application of HMFP in the biomedical and tissue engineering fields.

摘要

贻贝足蛋白是一种具有潜在生物医学应用价值的生物粘附蛋白，但其溶解度有限且生物稳定性较差，阻碍了其广泛应用。在本研究中，采用逐步选择性酶消化法成功提取了高溶解性的贻贝足蛋白（HMFP），其分子量在11-17 kDa范围内。此外，还合成了HMFP的双功能聚乙二醇（PEG）衍生物，命名为HMFP-PEG。傅里叶变换红外光谱（FTIR）分析证实了PEG对HMFP的成功修饰，而热重分析（TGA）和扫描电子显微镜（SEM）观察表明，PEG修饰显著提高了蛋白质的稳定性。HMFP和HMFP-PEG均能有效清除自由基，增强抗氧化酶活性，并降低丙二醛（MDA）水平。此外，它们还激活了磷脂酰肌醇-3激酶（PI3K）/蛋白激酶B（Akt）和核因子E2相关因子2（Nrf2）/血红素加氧酶-1（HO-1）信号通路，抑制过氧化氢（HO）诱导的细胞凋亡。值得注意的是，与HMFP相比，HMFP-PEG表现出更优异的抗氧化和细胞保护作用，表明PEG修饰提高了蛋白质的功能稳定性。本研究为HMFP在生物医学和组织工程领域的潜在应用提供了理论支持。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/88df03c02d59/antioxidants-14-00644-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/69503802c7cc/antioxidants-14-00644-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/61c6832f17fa/antioxidants-14-00644-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/296347ced214/antioxidants-14-00644-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/5cf8858ce212/antioxidants-14-00644-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/6add3c77e9d4/antioxidants-14-00644-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/6907c02a1830/antioxidants-14-00644-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/dac56606261a/antioxidants-14-00644-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/22a796aa33b2/antioxidants-14-00644-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/7122590be180/antioxidants-14-00644-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/34c30007aef0/antioxidants-14-00644-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/88df03c02d59/antioxidants-14-00644-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/69503802c7cc/antioxidants-14-00644-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/61c6832f17fa/antioxidants-14-00644-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/296347ced214/antioxidants-14-00644-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/5cf8858ce212/antioxidants-14-00644-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/6add3c77e9d4/antioxidants-14-00644-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/6907c02a1830/antioxidants-14-00644-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/dac56606261a/antioxidants-14-00644-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/22a796aa33b2/antioxidants-14-00644-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/7122590be180/antioxidants-14-00644-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/34c30007aef0/antioxidants-14-00644-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d871/12189154/88df03c02d59/antioxidants-14-00644-g011.jpg

相似文献

1
Highly Soluble Mussel Foot Protein Enhances Antioxidant Defense and Cytoprotection via PI3K/Akt and Nrf2/HO-1 Pathways.高溶解性贻贝足蛋白通过PI3K/Akt和Nrf2/HO-1信号通路增强抗氧化防御和细胞保护作用。
Antioxidants (Basel). 2025 May 27;14(6):644. doi: 10.3390/antiox14060644.
2
Dendrobium officinale extract alleviates aging-induced kidney injury by inhibiting oxidative stress via the PI3K/Akt/Nrf2/HO-1 pathway.铁皮石斛提取物通过PI3K/Akt/Nrf2/HO-1途径抑制氧化应激，减轻衰老诱导的肾损伤。
J Ethnopharmacol. 2025 Jun 19;352:120156. doi: 10.1016/j.jep.2025.120156.
3
Adefovir dipivoxil and pegylated interferon alfa-2a for the treatment of chronic hepatitis B: a systematic review and economic evaluation.阿德福韦酯与聚乙二醇化干扰素α-2a治疗慢性乙型肝炎：系统评价与经济学评估
Health Technol Assess. 2006 Aug;10(28):iii-iv, xi-xiv, 1-183. doi: 10.3310/hta10280.
4
Osmotic and stimulant laxatives for the management of childhood constipation.用于治疗儿童便秘的渗透性和刺激性泻药。
Cochrane Database Syst Rev. 2016 Aug 17;2016(8):CD009118. doi: 10.1002/14651858.CD009118.pub3.
5
Osmotic and stimulant laxatives for the management of childhood constipation.用于治疗儿童便秘的渗透性和刺激性泻药。
Cochrane Database Syst Rev. 2012 Jul 11(7):CD009118. doi: 10.1002/14651858.CD009118.pub2.
6
Home treatment for mental health problems: a systematic review.心理健康问题的居家治疗：一项系统综述
Health Technol Assess. 2001;5(15):1-139. doi: 10.3310/hta5150.
7
SUMOylation Inhibitors Exert a Protective Effect on Oxidative Damage in Retinal Pigment Epithelial Cells Through the Keap1/Nrf2/ARE Signaling Pathway.SUMO化抑制剂通过Keap1/Nrf2/ARE信号通路对视网膜色素上皮细胞的氧化损伤发挥保护作用。
Curr Mol Med. 2025 Jan 2. doi: 10.2174/0115665240350793241214050904.
8
Interventions for preventing weight gain after smoking cessation.戒烟后预防体重增加的干预措施。
Cochrane Database Syst Rev. 2012 Jan 18;1:CD006219. doi: 10.1002/14651858.CD006219.pub3.
9
Signs and symptoms to determine if a patient presenting in primary care or hospital outpatient settings has COVID-19.在基层医疗机构或医院门诊环境中，如果患者出现以下症状和体征，可判断其是否患有 COVID-19。
Cochrane Database Syst Rev. 2022 May 20;5(5):CD013665. doi: 10.1002/14651858.CD013665.pub3.
10
Intravenous magnesium sulphate and sotalol for prevention of atrial fibrillation after coronary artery bypass surgery: a systematic review and economic evaluation.静脉注射硫酸镁和索他洛尔预防冠状动脉搭桥术后房颤：系统评价与经济学评估
Health Technol Assess. 2008 Jun;12(28):iii-iv, ix-95. doi: 10.3310/hta12280.

引用本文的文献

1
Highly Soluble Mussel Foot Protein and Its Derivatives Inhibit Inflammation by Targeting NF-κB/PI3K-Akt Signaling and Promoting M2 Macrophage Polarization.高溶解性贻贝足蛋白及其衍生物通过靶向NF-κB/PI3K-Akt信号通路并促进M2巨噬细胞极化来抑制炎症。
Antioxidants (Basel). 2025 Aug 21;14(8):1021. doi: 10.3390/antiox14081021.

本文引用的文献

1
Mussel-Inspired Hydrogel Applied to Wound Healing: A Review and Future Prospects.贻贝启发水凝胶在伤口愈合中的应用：综述与未来展望
Biomimetics (Basel). 2025 Mar 26;10(4):206. doi: 10.3390/biomimetics10040206.
2
Role of the PI3K/AKT signaling pathway in the cellular response to Tumor Treating Fields (TTFields).PI3K/AKT信号通路在细胞对肿瘤治疗电场（TTFields）反应中的作用。
Cell Death Dis. 2025 Mar 27;16(1):210. doi: 10.1038/s41419-025-07546-8.
3
Efficient production of recombinant hybrid mussel proteins with improved adhesion.
高效生产具有增强粘附性的重组杂交贻贝蛋白。
Int J Biol Macromol. 2025 Feb;289:139937. doi: 10.1016/j.ijbiomac.2025.139937. Epub 2025 Jan 15.
4
Natural polysaccharides combined with mussel-inspired adhesion for multifunctional hydrogels in wound hemostasis and healing: A review.天然多糖与贻贝启发的粘附作用相结合用于伤口止血和愈合的多功能水凝胶：综述
Int J Biol Macromol. 2024 Dec;282(Pt 3):136965. doi: 10.1016/j.ijbiomac.2024.136965. Epub 2024 Oct 28.
5
Advances in Mussel Adhesion Proteins and Mussel-Inspired Material Electrospun Nanofibers for Their Application in Wound Repair.贻贝黏附蛋白的研究进展及贻贝仿生材料静电纺纳米纤维在创伤修复中的应用
ACS Biomater Sci Eng. 2024 Oct 14;10(10):6097-6119. doi: 10.1021/acsbiomaterials.4c01378. Epub 2024 Sep 10.
6
Characterization of a novel antioxidant byssal protein from Mytilus coruscus foot.从贻贝足部提取的一种新型抗氧化性贻贝蛋白的特性研究。
Int J Biol Macromol. 2024 Jul;273(Pt 2):133095. doi: 10.1016/j.ijbiomac.2024.133095. Epub 2024 Jun 10.
7
Salvianolic acid A alleviates HO-induced endothelial oxidative injury via miR-204-5p.丹酚酸 A 通过 miR-204-5p 减轻 HO 诱导的内皮氧化损伤。
Sci Rep. 2024 May 24;14(1):11931. doi: 10.1038/s41598-024-62556-4.
8
Protective Effect of Resveratrol Combined with Levodopa Against Oxidative Damage in Dopaminergic Neurons.白藜芦醇联合左旋多巴对多巴胺能神经元氧化损伤的保护作用。
Cell Biochem Biophys. 2024 Jun;82(2):817-826. doi: 10.1007/s12013-024-01233-9. Epub 2024 Apr 15.
9
Hypoxia, oxidative stress, and the interplay of HIFs and NRF2 signaling in cancer.缺氧、氧化应激以及 HIFs 和 NRF2 信号通路在癌症中的相互作用。
Exp Mol Med. 2024 Mar;56(3):501-514. doi: 10.1038/s12276-024-01180-8. Epub 2024 Mar 1.
10
PI3K/AKT/mTOR signaling transduction pathway and targeted therapies in cancer.PI3K/AKT/mTOR 信号转导通路与癌症的靶向治疗。
Mol Cancer. 2023 Aug 18;22(1):138. doi: 10.1186/s12943-023-01827-6.