使用多克隆抗血清在薄层板上检测血型糖鞘脂抗原。
Detection of blood group type glycosphingolipid antigens on thin-layer plates using polyclonal antisera.
作者信息
Hansson G C, Karlsson K A, Larson G, Samuelsson B E, Thurin J, Bjursten L M
出版信息
J Immunol Methods. 1985 Oct 24;83(1):37-42. doi: 10.1016/0022-1759(85)90055-9.
PMID:4056402
Abstract
The conditions for binding of antibodies to glycosphingolipids separated on a thin-layer plate have been optimized for polyclonal antisera. The method has a broad detection range with low background staining. Examples are shown for the detection of blood group A and B active glycosphingolipids.
摘要
针对多克隆抗血清，已优化了抗体与在薄层层析板上分离的糖鞘脂结合的条件。该方法检测范围广，背景染色低。展示了检测A、B血型活性糖鞘脂的示例。
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