Bennun F R, Roth G A, Monferran C G, Cumar F A
Departamento de Quimica Biologica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, CIQUIBIC-CONICET, Argentina.
Infect Immun. 1989 Mar;57(3):969-74. doi: 10.1128/iai.57.3.969-974.1989.
A search for compounds from intestinal mucosa of pigs carrying and not carrying blood group A-active substances (A+ and A- pigs, respectively) capable of binding cholera toxin (CT) was performed. Glycolipid extracts from a pool of pig intestinal mucosa resolved in thin-layer chromatography (TLC) revealed the presence of six to eight compounds capable of binding 125I-CT, two of them running as the ganglioside standards GM1 and GD1b. When intestinal mucosa glycolipids from single pigs were assayed by TLC for CT-binding capacity, two different patterns of labeling were observed. The main difference was at the level of compounds running below GD1b. The A+ pigs but not the A- pigs showed CT binding at this level. The major CT-binding compound detected only in A+ pigs was purified and some properties were determined. After TLC developed with different solvent systems, the purified compound bound CT and also immunoreacted with anti-A and anti-AB antisera but not with anti-B antiserum. The compound was also able to inhibit the hemagglutination of human A erythrocytes caused by anti-A antiserum, but inhibition was not observed with the B-anti-B or O (H)-Ulex europaeus lectin systems. A partial chemical characterization indicated that the active compound is a neutral glycosphingolipid containing glucose, fucose, galactose, and hexosamine. The existence of a blood group-active substance(s) able to interact with CT may help to explain the relationship between ABO blood groups and the diarrheal disease caused by infection with Vibrio cholerae.
对携带和不携带血型A活性物质的猪（分别为A+和A-猪）的肠黏膜中能够结合霍乱毒素（CT）的化合物进行了搜索。在薄层色谱（TLC）中分离的猪肠黏膜提取物中的糖脂显示存在6至8种能够结合125I-CT的化合物，其中两种的迁移情况与神经节苷脂标准品GM1和GD1b相同。当通过TLC检测单头猪的肠黏膜糖脂的CT结合能力时，观察到两种不同的标记模式。主要差异在于迁移至GD1b下方的化合物水平。A+猪而非A-猪在此水平显示出CT结合。仅在A+猪中检测到的主要CT结合化合物被纯化并测定了一些性质。在用不同溶剂系统展开的TLC之后，纯化的化合物结合CT并且还与抗A和抗AB抗血清发生免疫反应，但不与抗B抗血清反应。该化合物还能够抑制抗A抗血清引起的人A红细胞的血凝，但在B-抗B或O（H）-荆豆凝集素系统中未观察到抑制作用。部分化学表征表明活性化合物是一种含有葡萄糖、岩藻糖、半乳糖和己糖胺的中性糖鞘脂。能够与CT相互作用的血型活性物质的存在可能有助于解释ABO血型与霍乱弧菌感染引起的腹泻病之间的关系。
