Liu Yumeng, Liu Yuanyi, Bai Dongyi, Dugarjaviin Manglai, Zhang Xinzhuang
Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
Animals (Basel). 2025 Jun 9;15(12):1711. doi: 10.3390/ani15121711.
Skeletal muscle satellite cells are muscle stem cells that play an important role in the growth, development, and repair of skeletal muscle as well as in the locomotor performance of the animal body. Lysine is the first limiting amino acid and is involved in multiple metabolic pathways in the organism to maintain overall physiological requirements. In this study, Mongolian horse satellite cells were cultured using lysine culture solution at different concentrations, and the proliferative capacity of satellite cells was detected by the cck-8 assay, and the optimal culture concentration was selected. Then, whole transcriptome sequencing technology was used to determine the differential gene expression and regulatory pathways during the proliferation of lysine-cultured satellite cells after 48 h of culture. Our findings revealed that 0.5 mmol/L lysine is the optimal concentration to increase satellite cell activity in equine muscle. The differential genes involved in satellite cell proliferation were mainly enriched in the cAMPsignaling pathway, calcium signaling pathway, and PPAR signaling pathway. Furthermore, upregulation of , , and and downregulation of regulated the expression of the PPAR signaling pathway. 0.5 mmol/L lysine was the optimal concentration to increase satellite cell activity. Lysine can regulate mitochondrial function and lipid metabolism through the PPAR signaling pathway, and promote the proliferation of equine myosatellite cells.
骨骼肌卫星细胞是肌肉干细胞，在骨骼肌的生长、发育、修复以及动物体的运动表现中发挥着重要作用。赖氨酸是第一限制性氨基酸，参与机体多种代谢途径以维持整体生理需求。在本研究中，使用不同浓度的赖氨酸培养液培养蒙古马卫星细胞，通过cck - 8法检测卫星细胞的增殖能力，并选择最佳培养浓度。然后，利用全转录组测序技术确定培养48小时后赖氨酸培养的卫星细胞增殖过程中的差异基因表达及调控途径。我们的研究结果表明，0.5 mmol/L赖氨酸是提高马肌肉中卫星细胞活性的最佳浓度。参与卫星细胞增殖的差异基因主要富集在cAMP信号通路、钙信号通路和PPAR信号通路。此外， 、 和 的上调以及 的下调调节了PPAR信号通路的表达。0.5 mmol/L赖氨酸是提高卫星细胞活性的最佳浓度。赖氨酸可通过PPAR信号通路调节线粒体功能和脂质代谢，促进马肌卫星细胞的增殖。
