Uriostegui Escoto Daniel, Méndez de la Cruz Fausto Roberto, García Lorenzana Mario, López Ramírez Yolanda, Arenas Ríos Edith
Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, Iztapalapa, Ciudad de México C.P. 09340, Mexico.
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México, A.P. 70515, Coyoacán C.P. 04510, Mexico.
Animals (Basel). 2025 Jun 17;15(12):1784. doi: 10.3390/ani15121784.
The global increase in temperature has directly impacted the environment and reproduction of ectothermic organisms, leading to defects in processes that intervene in sperm quality and maturation. The present study evaluates the effect of temperature on sperm quality of the lizard in the surroundings of Landa de Matamoros, Querétaro, México. As a species under special protection, only 12 male individuals were captured in December 2020 with 4 exposed at (1) preferred temperature (Tpref) at 24 °C; (2) low temperature (LT) at 21 °C; (3) either individuals without treatment (Control) with fluctuating temperature of the habituation site, to compare the effect on the viability, concentration, and integrity of DNA, as well as the presence of the cytoplasmic droplet (CD). Sperm viability decreased by approximately 50% in organisms exposed to Tpref, accompanied by a significant reduction in concentration. Likewise, the CD evaluation showed a retention percentage of 30% at the cauda level, while the LT and Control groups showed 5% in this terminal area of the epididymis. The percentage of sperm with intact DNA was also decreased in the Tpref group compared to the LT and control groups. These findings highlight how thermal environments impact reptilian reproduction, reinforcing not only what has already been found in these species in recent years, but also the repercussions that it presents in the medium- or long-term exposure in these organisms at an ecological level, as well as the consequences with the potential damage and even infertility, in case of prolonged exposition to preferred temperatures.
全球气温上升直接影响了变温生物的环境和繁殖，导致参与精子质量和成熟过程出现缺陷。本研究评估了温度对墨西哥克雷塔罗州兰达德马塔莫罗斯周边地区蜥蜴精子质量的影响。作为一种受特殊保护的物种，2020年12月仅捕获了12只雄性个体，其中4只分别暴露于：(1) 24°C的偏好温度（Tpref）；(2) 21°C的低温（LT）；(3) 处于适应环境温度波动的未处理个体（对照），以比较对DNA活力、浓度和完整性以及细胞质滴（CD）存在情况的影响。暴露于Tpref的生物体中精子活力下降了约50%，同时浓度显著降低。同样，CD评估显示在尾部水平的保留率为30%，而LT组和对照组在附睾的这个末端区域显示为5%。与LT组和对照组相比，Tpref组中DNA完整的精子百分比也有所下降。这些发现突出了热环境如何影响爬行动物的繁殖，不仅强化了近年来在这些物种中已发现的情况，还强调了在生态层面这些生物体中长期暴露所带来的影响，以及长期暴露于偏好温度下可能造成的潜在损害甚至不育的后果。
