Nuñez Suleivys M, García Ahyra, Roman Tanya, Aguilar Luis, Tarnok María Elena, Guzmán Fanny, Cárdenas Constanza, Ponce Sebastián, Vásquez Dreidy, Carrasco Samuel, Valín José Luis
Escuela de Ingeniería Química, Pontificia Universidad Católica de Valparaíso, Valparaíso 2340025, Chile.
Instituto de Química, Pontificia Universidad Católica de Valparaíso, Valparaíso 2373223, Chile.
Int J Mol Sci. 2025 Jun 19;26(12):5877. doi: 10.3390/ijms26125877.
In the search for safe and effective natural antioxidants, this study investigates the antioxidant and cytotoxic properties of aqueous extracts obtained from three fungi of the family : sp., sp., and , all associated with endemic trees of the Chilean sclerophyll forest. Antioxidant capacity was assessed through DPPH, ABTS, and hydroxyl radical scavenging assays. sp. exhibited the highest antioxidant activity across all methods, which was consistent with its elevated polyphenol content. , on the other hand, had the highest protein concentration but comparatively lower antioxidant activity. Cytotoxicity was evaluated using the WST-1 assay in the RTgill-W1 salmonid cell line, revealing that sp. displayed the lowest cytotoxicity at both tested concentrations, suggesting it may be suitable for bioactive applications in aquaculture. In contrast, sp. and showed significant cytotoxic effects at higher concentrations. These findings highlight the potential of sp. as a natural antioxidant with low cytotoxicity and encourages further exploration of native forest fungi as sources of functional bioactive compounds for food, nutraceutical, or aquaculture applications.
在寻找安全有效的天然抗氧化剂的过程中，本研究调查了从三个真菌家族获得的水提取物的抗氧化和细胞毒性特性： 种、 种和 种，它们都与智利硬叶林的特有树木有关。通过DPPH、ABTS和羟基自由基清除试验评估抗氧化能力。 种在所有方法中均表现出最高的抗氧化活性，这与其较高的多酚含量一致。另一方面， 种的蛋白质浓度最高，但抗氧化活性相对较低。使用WST-1试验在RTgill-W1鲑鱼细胞系中评估细胞毒性，结果表明 种在两个测试浓度下均表现出最低的细胞毒性，表明它可能适用于水产养殖中的生物活性应用。相比之下， 种和 种在较高浓度下表现出显著的细胞毒性作用。这些发现突出了 种作为一种具有低细胞毒性的天然抗氧化剂的潜力，并鼓励进一步探索本地森林真菌作为食品、营养保健品或水产养殖应用中功能性生物活性化合物的来源。
