Li Nanxin, Li Chao, Li Dongbo, Abbas Awn, Chen Xingyu, Ai Xiaoyang, Zhang Wei, Shu Gang, Lin Juchun, Li Haohuan, Xu Funeng, Peng Guangneng, Fu Hualin
Department of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
Int J Mol Sci. 2025 Jun 19;26(12):5908. doi: 10.3390/ijms26125908.
Wound infections remain significant challenges for current tissue adhesives, primarily due to their poor adhesion in moist environments, slow bonding, cytotoxicity, and limited antibacterial properties. Slightly acidic electrolyzed water (SAEW), a potent disinfectant, suffers from limited stability due to chlorine loss. This study developed a novel SAEW-based hydrogel (SAEW-gel) by combining SAEW with chitosan and β-glycerol disodium phosphate to improve its stability and therapeutic potential. SAEW-gel demonstrated high water absorption, long-term water retention, and enhanced antibacterial activity against and compared to SAEW alone. It maintained germicidal efficacy after prolonged storage and significantly accelerated wound healing in a rat model, achieving a 95.41% healing rate by the 12th day of treatment. Mechanistically, SAEW-gel reduced inflammatory cell infiltration, promoted granulation and collagen formation, and regulated inflammatory markers (IL-6, IL-1β, TNF-α, MPO, HYP). These findings highlight SAEW-gel as a promising biomaterial for treating infectious wounds and support its potential for future clinical application.
伤口感染仍然是当前组织粘合剂面临的重大挑战，主要原因在于它们在潮湿环境中粘附性差、粘结速度慢、具有细胞毒性且抗菌性能有限。微酸性电解水（SAEW）作为一种有效的消毒剂，由于氯的损失而稳定性有限。本研究通过将SAEW与壳聚糖和β -甘油磷酸二钠结合，开发了一种新型的基于SAEW的水凝胶（SAEW -凝胶），以提高其稳定性和治疗潜力。与单独的SAEW相比，SAEW -凝胶表现出高吸水性、长期保水性以及对[具体细菌1]和[具体细菌2]增强的抗菌活性。它在长时间储存后仍保持杀菌效果，并在大鼠模型中显著加速伤口愈合，在治疗第12天时愈合率达到95.41%。从机制上讲，SAEW -凝胶减少了炎症细胞浸润，促进了肉芽组织和胶原蛋白形成，并调节了炎症标志物（IL - 6、IL - 1β、TNF -α、MPO、HYP）。这些发现突出了SAEW -凝胶作为治疗感染性伤口的一种有前景的生物材料，并支持其未来临床应用的潜力。
