Maneeprasert Pariya, Thaisakun Siriwan, Thanananta Theerachai, Thanananta Narumol, Lokkamlue Noppamart, Mongkolsiriwatana Chareerat
Interdisciplinary Graduate Program in Genetic Engineering and Bioinformatics, The Graduate School, Kasetsart University, 50 Ngamwongwan, Chatuchak, Bangkok 10900, Thailand.
National Center for Genetic Engineering and Biotechnology, National Science and Technology Develoment Agency, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
Genes (Basel). 2025 Jun 18;16(6):718. doi: 10.3390/genes16060718.
The flowering transition is a critical process determining the onset of reproductive development and fruit production. The molecular mechanisms underlying this process in coconuts are poorly understood; however, recent studies have identified as a potential regulator of the floral transition in coconuts.
In this study, we characterized the molecular structure of and analyzed its alternative splicing forms in tall and dwarf varieties of coconut palms during the flowering transition. We used qRT-PCR to measure the expression levels of at different developmental stages.
was expressed in leaves and the shoot apical meristem (SAM) during the flowering transition in both coconut varieties and flower tissues during flower development. Interestingly, the expression levels of complex isoforms of were higher in the leaves of dwarf coconuts than in those of tall coconuts, suggesting their involvement in shortening the vegetative growth phase of dwarf coconuts. The gene structure of was found to be conserved across different plant species, indicating the evolutionary conservation of the floral transition process.
Our findings provide insight into the molecular mechanisms underlying the floral transition and flower development processes in coconut palm. The tissue-specific expression patterns of isoforms show their potential roles in growth and development. Further investigations focusing on the functional characterization of isoforms will have practical implications for coconut breeding and cultivation strategies.
开花转变是决定生殖发育起始和果实生产的关键过程。人们对椰子中这一过程的分子机制了解甚少；然而，最近的研究已将[具体基因名称未给出]确定为椰子开花转变的潜在调节因子。
在本研究中，我们对[具体基因名称未给出]的分子结构进行了表征，并分析了其在开花转变期间椰子高种和矮种品种中的可变剪接形式。我们使用qRT-PCR来测量[具体基因名称未给出]在不同发育阶段的表达水平。
在两个椰子品种的开花转变期间，[具体基因名称未给出]在叶片和茎尖分生组织（SAM）中表达，在花发育期间在花组织中也有表达。有趣的是，[具体基因名称未给出]复杂异构体的表达水平在矮种椰子的叶片中高于高种椰子，这表明它们参与缩短矮种椰子的营养生长阶段。发现[具体基因名称未给出]的基因结构在不同植物物种中是保守的，这表明开花转变过程的进化保守性。
我们的研究结果为椰子开花转变和花发育过程的分子机制提供了见解。[具体基因名称未给出]异构体的组织特异性表达模式显示了它们在生长和发育中的潜在作用。进一步针对[具体基因名称未给出]异构体功能表征的研究将对椰子育种和栽培策略具有实际意义。
