Naz R K, Littman B A, Stillman R J
J In Vitro Fert Embryo Transf. 1985 Sep;2(3):143-5. doi: 10.1007/BF01131501.
Frozen-thawed husband's semen was utilized for insemination of spouse's oocytes in vitro. Postthaw semen had a low motility (40%) with a poor forward progression (+2), which subsequently decreased rapidly in the regularly used Ham's F-10 medium (pH 7.42, 280 mosmol/kg) supplemented with 7.5% fetal cord serum. When Ham's F-10 was supplemented with an additional 0.5 mmol each of magnesium sulfate (MgSO4) and potassium bicarbonate (KHCO3) at a higher pH (pH 7.69, 280 mosmol/kg), the sperm maintained their motility and forward progression and fertilized all the oocytes, which subsequently cleaved and developed into normal embryos. Transfer of these embryos resulted in a normal pregnancy. Our results indicate that cryopreserved semen of poor quality can be successfully employed for an in vitro fertilization-embryo transfer (IVF-ET) program, especially when MgSO4 and KHCO3 are used in Ham's F-10 at a higher pH.
解冻后的丈夫精液被用于对配偶的卵母细胞进行体外授精。解冻后的精液活力较低（40%），前向运动能力较差（+2级），随后在添加了7.5%胎牛血清的常用哈姆F-10培养基（pH 7.42，280毫渗摩尔/千克）中迅速下降。当哈姆F-10培养基在较高pH值（pH 7.69，280毫渗摩尔/千克）下额外添加0.5毫摩尔硫酸镁（MgSO4）和碳酸氢钾（KHCO3）时，精子保持了活力和前向运动能力，并使所有卵母细胞受精，这些卵母细胞随后分裂并发育成正常胚胎。移植这些胚胎导致了正常妊娠。我们的结果表明，质量较差的冷冻精液可成功用于体外受精-胚胎移植（IVF-ET）程序，尤其是当在较高pH值下在哈姆F-10培养基中使用硫酸镁和碳酸氢钾时。
