Mohapi Dineo Attela, Ramatla Tsepo, Thekisoe Oriel, Khetsha Zenzile Peter, Nkhebenyane Jane
Centre for Applied Food Safety and Biotechnology, Department of Life Sciences, Central University of Technology, 1 Park Road, Bloemfontein 9300, South Africa.
Unit for Environmental Sciences and Management, North-West University, Potchefstroom 2520, South Africa.
Foods. 2025 Jun 19;14(12):2139. doi: 10.3390/foods14122139.
The important role of antibiotics cannot be overestimated, as human health relies heavily on them for the treatment of infectious diseases. In this study, the antimicrobial susceptibility profiles of pathogens isolated from spinach () and cabbage () collected from Free State Province were investigated. A total of 38 isolates representing 10 species, (5.3%), (13.2%), (5.3%), (5.3%), (5.3%), (15.8%), (15.8%), (18.4%), (5.3%), and (10.5%), were confirmed by the Analytical Profile Index (API). We evaluated antibiotic resistance patterns of 38 unduplicated isolates using the disk diffusion method. As a result, (18.4%), (15.8%), (15.8%), (13.2%), and (10.5%), as well as 5.3% each for , , , , and showed resistance to tested antibiotics. The majority (84%) of the isolates showed resistance to tetracycline, and penicillin had a value of 71%. A total of 79% of the antibiotic-resistant isolates demonstrated multidrug resistance (MDR) to several classes such as -lactams, chloramphenicol, tetracycline, aminoglycosides, and macrolides. The results highlight the importance of monitoring the microbiological quality of leafy greens as they contain antibiotic-resistant bacteria that could affect human health when consumed.
抗生素的重要作用再怎么高估都不为过，因为人类健康在很大程度上依赖它们来治疗传染病。在本研究中，对从自由邦省采集的菠菜（）和卷心菜（）中分离出的病原体的抗菌药敏谱进行了调查。通过分析谱指数（API）确认了总共38株分离株，代表10个物种，（5.3%），（13.2%），（5.3%），（5.3%），（5.3%），（15.8%），（15.8%），（18.4%），（5.3%）和（10.5%）。我们使用纸片扩散法评估了38株无重复分离株的抗生素耐药模式。结果显示，（18.4%），（15.8%），（15.8%），（13.2%）和（10.5%），以及，，，和各5.3%的分离株对测试抗生素表现出耐药性。大多数（84%）分离株对四环素耐药，青霉素的耐药率为71%。总共79%的耐药分离株对几类抗生素如β-内酰胺类、氯霉素、四环素、氨基糖苷类和大环内酯类表现出多重耐药（MDR）。结果突出了监测绿叶蔬菜微生物质量的重要性，因为它们含有抗生素耐药细菌，食用时可能会影响人类健康。
