从绵羊和山羊中分离的产志贺毒素和产超广谱β-内酰胺酶（ESBL）大肠杆菌的分子检测。

Molecular detection of Shiga toxin and extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates from sheep and goats.

机构信息

Unit for Environmental Sciences and Management, North West University, Potchefstroom, 2531, South Africa.

出版信息

Mol Biol Rep. 2024 Jan 2;51(1):57. doi: 10.1007/s11033-023-08987-0.

DOI:10.1007/s11033-023-08987-0

PMID:38165462

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10761393/

Abstract

BACKGROUND

The Shiga toxin (Stx)-producing Escherichia coli (STEC) have become important global public health concerns. This study investigated the prevalence, antimicrobial resistance profile, and extended-spectrum beta-lactamase-producing E. coli in sheep and goat faeces.

METHODS AND RESULTS

A total of 53 E. coli isolates were confirmed by PCR targeting the uidA [β-D glucuronidase] gene. The Shiga toxin genes stx1 and stx2, as well as bfpA, vir, eaeA, lt and aafII virulence genes, were detected in this study. Of the 53 isolates confirmed to be STEC, 100% were positive for stx2 and 47.2% for stx1. Three isolates possessed a combination of stx1 + stx2 + eaeA, while four isolates harboured stx1 + stx2 + vir virulence genes. The isolates displayed phenotypic antimicrobial resistance against erythromycin (66.04%), colistin sulphate (43.4%), chloramphenicol (9.4%) and ciprofloxacin (1.9%). A total of 28.8% of the strains were phenotypically considered ESBL producers and contained the beta-lactamase bla and bla gene groups. A larger proportion of the E. coli strains (86.8%) contained the antibiotic sulphonamide resistant (sulII) gene, while 62.3%, 62.3%, 52.8%, 43.4%, 41.5%, 20.8%, 18.9%, 11.3%, 11.3%, 9.4%, 9.4% and 5.7% possessed mcr-4, floR, mcr-1, tet(A), sulI, tet(O), tet(W), parC, mcr-2, ampC 5, qnrS and ermB genes, respectively. Thirteen isolates of the ESBL-producing E. coli were considered multi-drug resistant (MDR). One Shiga toxin (stx2) and two beta-lactamase genes (bla and bla groups) were present in 16 isolates. In conclusion, the E. coli isolates from the small stock in this study contained a large array of high antibiotic resistance and virulence profiles.

CONCLUSIONS

Our findings highlight the importance of sheep and goats as sources of virulence genes and MDR E. coli. From a public health and veterinary medicine perspective, the characterization of ESBL producers originating from small livestock (sheep and goats) is crucial due to their close contact with humans.

摘要

背景

产志贺毒素（Stx）大肠杆菌（STEC）已成为重要的全球公共卫生关注问题。本研究调查了绵羊和山羊粪便中产志贺毒素大肠杆菌的流行情况、抗生素耐药谱和产超广谱β-内酰胺酶大肠杆菌。

方法和结果

通过针对 uidA[β-D 葡糖醛酸酶]基因的 PCR 共确认了 53 株大肠杆菌。本研究中检测到了志贺毒素基因 stx1 和 stx2 以及 bfpA、vir、eaeA、lt 和 aafII 毒力基因。在这 53 株被确认为 STEC 的分离株中，100%为 stx2 阳性，47.2%为 stx1 阳性。3 株分离株携带 stx1+stx2+eaeA 组合，4 株分离株携带 stx1+stx2+vir 毒力基因。分离株对红霉素（66.04%）、硫酸粘菌素（43.4%）、氯霉素（9.4%）和环丙沙星（1.9%）表现出表型抗生素耐药性。共有 28.8%的菌株被认为是表型产超广谱β-内酰胺酶（ESBL），并含有β-内酰胺酶 bla 和 bla 基因群。更大比例的大肠杆菌菌株（86.8%）含有抗生素磺胺类耐药（sulII）基因，而 62.3%、62.3%、52.8%、43.4%、41.5%、20.8%、18.9%、11.3%、11.3%、9.4%、9.4%和 5.7%分别携带 mcr-4、floR、mcr-1、tet(A)、sulI、tet(O)、tet(W)、parC、mcr-2、ampC 5、qnrS 和 ermB 基因。13 株产 ESBL 的大肠杆菌被认为是多药耐药（MDR）。16 株分离株中存在 1 种志贺毒素（stx2）和 2 种β-内酰胺酶基因（bla 和 bla 基因群）。总之，本研究中小畜（绵羊和山羊）粪便中的大肠杆菌分离株含有大量的高抗生素耐药性和毒力特征。从公共卫生和兽医医学的角度来看，由于与人类密切接触，从小型家畜（绵羊和山羊）中分离的 ESBL 产生菌的特征至关重要。