Lučev Milica, Stepanović Jelena, Kandić Raftery Vesna, Čamdzija Zoran, Obradović Ana, Stevanović Milan, Stanković Slavica
Maize Research Institute Zemun Polje, Slobodana Bajića 1, 11185 Belgrade, Serbia.
Institute of Pesticides and Environmental Protection, Banatska 31b, 11080 Belgrade, Serbia.
Foods. 2025 Jun 19;14(12):2146. doi: 10.3390/foods14122146.
The presence of filamentous fungi with toxigenic ability from the genera is frequently found in maize kernels, and this can lead to decay and mycotoxin contamination of the kernels. In this study, we morphologically and molecularly characterized 45 isolates of section originating from maize and small grains (wheat, triticale, and spelt) in Serbia. Based on morphological traits, they were classified into two morpho groups. Representative isolates from both morpho groups were further molecularly characterized through sequencing of ITS, CaM and RPB2 genes in order to compare species composition, which could affect specific mycotoxicological risks. Morpho GroupI was molecularly identified as and morpho GroupII as . Phylogenetic analysis of the CaM gene revealed that the Serbian isolate belongs to the H8 haplotype, while isolates clustered into two subclusters. This is the first report of as the causal agent of black mold of small grains (wheat, triticale and spelt) in Serbia. This distribution underscores the ecological preferences of species within the genus Section across various agricultural products. It emphasizes the importance of comprehending their occurrence, distribution, aggressiveness and potential for mycotoxin production in food safety assessments.
具有产毒能力的该属丝状真菌经常在玉米粒中被发现，这可能导致玉米粒腐烂和霉菌毒素污染。在本研究中，我们对来自塞尔维亚玉米和小粒谷物（小麦、小黑麦和斯佩尔特小麦）的45株该组分离株进行了形态学和分子特征分析。基于形态特征，它们被分为两个形态组。为了比较可能影响特定霉菌毒素毒理学风险的物种组成，对两个形态组的代表性分离株通过ITS、CaM和RPB2基因测序进行了进一步的分子特征分析。形态组I在分子水平上被鉴定为，形态组II被鉴定为。对CaM基因的系统发育分析表明，塞尔维亚分离株属于H8单倍型，而分离株聚为两个亚簇。这是塞尔维亚首次报道作为小粒谷物（小麦、小黑麦和斯佩尔特小麦）黑霉病病原体。这种分布强调了该组内物种在各种农产品中的生态偏好。它强调了在食品安全评估中了解它们的发生、分布、侵袭性和产霉菌毒素潜力的重要性。
