Skinner D M, Chambers C A
Nucleic Acids Res. 1977 Jan;4(1):43-62. doi: 10.1093/nar/4.1.43.
The separated H- and L-strands of the alpha-satellite of the guinea pig, Cavea porcellus, recovered from centrifugation in alkaline CsC1 gradients, from complexes with 7 different double-stranded (ds) DNAs including those of 1 bacteriophage, 2 prokaryotes, 2 invertebrates and 2 mammals. The complexes are not artifacts due to in vitro labeling of the satellite, methods of collection, the presence of divalent cations, or the fact that trace amounts of single-stranded (ss) DNAs are used. More complex dsDNAs, such as that recovered from nicked RF M13, do not associate with dsDNAs.
豚鼠(Cavea porcellus)α卫星DNA的H链和L链在碱性CsC1梯度离心中分离后,与7种不同的双链(ds)DNA形成复合物,这些双链DNA包括1种噬菌体、2种原核生物、2种无脊椎动物和2种哺乳动物的DNA。这些复合物并非由于卫星DNA的体外标记、收集方法、二价阳离子的存在或使用痕量单链(ss)DNA而产生的假象。更复杂的dsDNA,如从切口RF M13中回收的dsDNA,不与dsDNA结合。