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卫星DNA单链与其他DNA的相互作用:1. 豚鼠α卫星与异源双链DNA结合的条件。

The interactions of the separated strands of satellite DNAs with other DNAs: 1. Conditions for associations of the alpha-satellite of the guinea pig with heterologous double-stranded DNAs.

作者信息

Skinner D M, Chambers C A

出版信息

Nucleic Acids Res. 1977 Jan;4(1):43-62. doi: 10.1093/nar/4.1.43.

Abstract

The separated H- and L-strands of the alpha-satellite of the guinea pig, Cavea porcellus, recovered from centrifugation in alkaline CsC1 gradients, from complexes with 7 different double-stranded (ds) DNAs including those of 1 bacteriophage, 2 prokaryotes, 2 invertebrates and 2 mammals. The complexes are not artifacts due to in vitro labeling of the satellite, methods of collection, the presence of divalent cations, or the fact that trace amounts of single-stranded (ss) DNAs are used. More complex dsDNAs, such as that recovered from nicked RF M13, do not associate with dsDNAs.

摘要

豚鼠(Cavea porcellus)α卫星DNA的H链和L链在碱性CsC1梯度离心中分离后,与7种不同的双链(ds)DNA形成复合物,这些双链DNA包括1种噬菌体、2种原核生物、2种无脊椎动物和2种哺乳动物的DNA。这些复合物并非由于卫星DNA的体外标记、收集方法、二价阳离子的存在或使用痕量单链(ss)DNA而产生的假象。更复杂的dsDNA,如从切口RF M13中回收的dsDNA,不与dsDNA结合。

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本文引用的文献

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SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.DNA大小与形状的沉降研究
J Mol Biol. 1965 Feb;11:373-90. doi: 10.1016/s0022-2836(65)80064-x.
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NUCLEOTIDE COMPOSITION OF SHORT SEGMENTS OF DNA MOLECULES.DNA分子短片段的核苷酸组成
J Mol Biol. 1965 Feb;11:223-37. doi: 10.1016/s0022-2836(65)80053-5.
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A satellite DNA isolated from human tissues.从人体组织中分离出的一种卫星DNA。
J Mol Biol. 1967 Feb 14;23(3):619-22. doi: 10.1016/s0022-2836(67)80130-x.

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