Olsen W L, Staudenbauer W L, Hofschneider P H
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2570-3. doi: 10.1073/pnas.69.9.2570.
Infection of the temperature-sensitive E. coli mutant HfrH 165/70 (dnaB) with the filamentous single-stranded DNA phage M13 is abortive at the restrictive temperature. Upon infection at 41 degrees , single-stranded phage DNA penetrates the cell and is converted in a rifampicin-sensitive step to the double-stranded replicative form (RF). The parental RF attaches to the cell membrane, but subsequent replication of the RF is blocked. It is concluded that in M13 infection semiconservative RF replication of a double strand to a double strand, in contrast to single-stranded DNA synthesis, depends specifically on the dnaB function.
温度敏感型大肠杆菌突变体HfrH 165/70（dnaB）被丝状单链DNA噬菌体M13感染后，在限制温度下会出现流产感染。在41摄氏度感染时，单链噬菌体DNA进入细胞，并在对利福平敏感的步骤中转化为双链复制型（RF）。亲代RF附着于细胞膜，但随后RF的复制被阻断。由此得出结论，在M13感染中，与单链DNA合成不同，双链到双链的半保留RF复制特别依赖于dnaB功能。
