豚鼠染色体中卫星DNA的定位。

Localization of satellite DNAs in the chromosomes of the guinea pig.

作者信息

Duhamel-Maestracci N, Simard R, Harbers K, Spencer J H

出版信息

Chromosoma. 1979 Oct 2;75(1):63-74. doi: 10.1007/BF00330625.

Abstract

The in situ hybridization method has been used to investigate the localization of each of the three satellite DNAs present in the genome of the guinea pig. Purified fractions of the satellite DNAs were utilized as templates for synthesis of 3H-labeled complementary RNA (cRNA) by E. coli RNA polymerase, then each cRNA was hybridized to metaphase spreads of embryonic guinea pig cells. The cRNAs of all three satellite DNAs hybridized predominantly to the centromeric region of the chromosomes. The cRNAs of satellite DNAs II and III hybridized to all chromosomes except the Y chromosome. The cRNA of satellite DNA I did not hybridize to the Y chromosome nor to two pairs of small acrocentric chromosomes. Satellite II cRNA hybridized to the telomeric region of chromosomes 3 and 4.

摘要

原位杂交方法已被用于研究豚鼠基因组中存在的三种卫星DNA各自的定位。将卫星DNA的纯化组分用作模板，通过大肠杆菌RNA聚合酶合成3H标记的互补RNA（cRNA），然后将每种cRNA与豚鼠胚胎细胞的中期染色体铺展进行杂交。所有三种卫星DNA的cRNA主要与染色体的着丝粒区域杂交。卫星DNA II和III的cRNA与除Y染色体外的所有染色体杂交。卫星DNA I的cRNA既不与Y染色体杂交，也不与两对小近端着丝粒染色体杂交。卫星II cRNA与3号和4号染色体的端粒区域杂交。