Liang Shengnan, Wang Xinyu, Zheng Tianhang, Liu Meishuai, Li Chun, Liu Libo, Zhang Guofang
College of Food Science, Northeast Agricultural University, Harbin 150030, China.
Heilongjiang Green Food Science Research Institute, Harbin 150028, China.
J Agric Food Chem. 2025 Jul 9;73(27):16673-16696. doi: 10.1021/acs.jafc.5c03079. Epub 2025 Jun 26.
Bioactive peptides (BPs) are widely used in the food industry, pharmaceutical industry, cosmetics industry, and feed industry due to their excellent biological activity, selectivity, and low toxicity. Despite the continuous emergence of articles on the functional research of BPs and the commercialization of BPs, there is a lack of a systematic review of functional evaluation methods for BPs. This article centers on BPs, systematically summarizing their major preparation methods and global regulatory frameworks. It delves into an analysis of their biological functions and the relationship between structure and function, in addition to outlining the degradation pathways of BPs and providing suggestions for improving their stability. Notably, for the first time, we have reviewed the functional evaluation systems applicable to BPs. This article proposes a comprehensive evaluation system for BPs, covering various aspects from computer simulation prediction to clinical application evaluation. It comprehensively explores the application value of computer technology, chemical analysis, cell models, animal models, and clinical evaluation in the functional evaluation of BPs and points out the limitations faced by each evaluation model. In addition, for the first time, the introduction of organoid and microfluidic technology into the functional evaluation model of the food industry has been proposed. This integrated bottom-up evaluation framework establishes a multidisciplinary paradigm for BP functional assessment, synergizing computational prediction, organoid-microfluidic validation, and clinical translation. It provides both methodological advancements for rational experimental design and actionable strategies to accelerate the development of BP-derived therapeutics and functional products, ultimately bridging the gap between academic discovery and industrial application.
生物活性肽（BPs）因其出色的生物活性、选择性和低毒性，在食品工业、制药工业、化妆品工业和饲料工业中得到广泛应用。尽管关于BPs功能研究的文章不断涌现，且BPs也实现了商业化，但目前缺乏对BPs功能评价方法的系统综述。本文围绕BPs展开，系统总结了其主要制备方法和全球监管框架。除了概述BPs的降解途径并提出提高其稳定性的建议外，还深入分析了它们的生物学功能以及结构与功能之间的关系。值得注意的是，我们首次对适用于BPs的功能评价体系进行了综述。本文提出了一个针对BPs的综合评价体系，涵盖从计算机模拟预测到临床应用评价的各个方面。全面探讨了计算机技术、化学分析、细胞模型、动物模型和临床评价在BPs功能评价中的应用价值，并指出了每个评价模型面临的局限性。此外，首次提出将类器官和微流控技术引入食品工业的功能评价模型。这种自下而上的综合评价框架为BP功能评估建立了多学科范式，将计算预测、类器官 - 微流控验证和临床转化协同起来。它既为合理的实验设计提供了方法学上的进步，也为加速BP衍生疗法和功能性产品的开发提供了可操作的策略，最终弥合学术发现与工业应用之间的差距。
