Laboratoire des Sciences Fondamentales (LSF), University Amar Telidji of Laghouat, BP. 37G (03000), Laghouat, Algeria.
Biomol Concepts. 2024 Feb 14;15(1). doi: 10.1515/bmc-2022-0040. eCollection 2024 Jan 1.
This study explores the synergistic antibacterial effects of essential oils (EOs) and phenolic extracts from three plants against foodborne pathogenic bacteria. The present work aimed to investigate the synergistic effects of the binary and the ternary combinations of extracts using different blend proportions of the following plant extracts: (AC), (AHA), and (CA). The antimicrobial activities of EOs and phenolic extracts were determined and evaluated against five strains. For the EOs, the results of the DIZ showed the existence of synergism for different combinations of binary blends, such as AC/AHA or AHA/CA against , and AC/CA against . In addition, ternary blends of AC:AHA:CA at a ratio of 1/6:2/3:1/6 exhibited a synergy effect, as measured by the CI, against . On the other hand, for the phenolic extracts, synergistic effects were noticed for binary blends of AC/CA at different ratios against , and strains. Similarly, ternary blends of phenolic extracts presented synergy against , , strains, and even . In this case, the blending ratios were crucial determining factors for maximizing the synergy effect. The study established that the proportion of a single drug could play an essential role in determining the bioefficacy of a drug combination treatment. Therefore, the results showed the importance of studying the modulation of antibacterial activities based on the proportions of extracts in the mixture and finding the range of proportions (as determined by SLMD) that have a synergistic/additive/antagonistic effect with no or low side effects, which can be used in a food preservation system.
本研究探讨了三种植物的精油（EOs）和酚类提取物对食源性病原体的协同抗菌作用。本工作旨在研究二元和三元混合物提取物的协同作用，使用以下植物提取物的不同混合比例：（AC）、（AHA）和（CA）。EOs 和酚类提取物的抗菌活性通过 DIZ 测定并针对五种菌株进行评估。对于 EOs，DIZ 的结果表明，不同二元混合物的组合存在协同作用，例如 AC/AHA 或 AHA/CA 对 ，以及 AC/CA 对 。此外，AC:AHA:CA 的三元混合物以 1/6:2/3:1/6 的比例显示出协同作用，CI 测量结果表明其对 表现出协同作用。另一方面，对于酚类提取物，在不同比例下，AC/CA 二元混合物对 、 和 菌株表现出协同作用。同样，酚类提取物的三元混合物对 、 、 菌株甚至 表现出协同作用。在这种情况下，混合比例是最大限度发挥协同作用的关键决定因素。该研究确定，单一药物的比例可能在确定药物组合治疗的生物疗效方面发挥重要作用。因此，结果表明，根据混合物中提取物的比例研究抗菌活性的调制以及找到具有协同/附加/拮抗作用且无或低副作用的比例范围（由 SLMD 确定）非常重要，这些比例范围可用于食品保存系统。
