Guo Yuhua, Li Yamei, Zhang Pengfei, Luo Zuliang, Yin Junmei, Ma Xiaojun, Yuan Chao
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Haikou 571101, China.
Plants (Basel). 2025 Jun 10;14(12):1767. doi: 10.3390/plants14121767.
() Lour., a medicinal species of the family, is used for medical purposes. Bornyl acetate, camphor, and borneol are the main bioactive ingredients in fruit, and the amount of bornyl acetate is regarded as a measure of the fruit's quality. In order to explore the major effective genes regulating the biosynthesis of camphane volatile terpenes in some DEGs involved in camphane volatile terpene biosynthesis and transcription factors were analyzed and summarized in this study. The result showed that the content of bornyl acetate was altered in the different growth stages. In particular, the significant change occurred from 7 to 30 DAP (days after pollination). The content of bornyl acetate at 30 DAP was 169.3% more than that at 7 DAP. In total, 4782 up-regulated and 5284 down-regulated unigenes were found in G2 vs. G1, as well as 3324 up-regulated and 5036 down-regulated unigenes in G3 vs. G1, and 3332 up-regulated and 4490 down-regulated unigenes in G3 vs. G2. A total of 323 up-regulated and 820 down-regulated unigenes were shared in three growth stage comparisons. We screened the genes that encode the enzymes most likely to inhibit bornyl diphosphate synthase, borneol dehydrogenase, and BAHD acyltransferases. Interestingly, we found that borneol dehydrogenase and bornyl diphosphate synthase displayed bi-substrate features, suggesting that a substrate of catalyzation is promiscuity in the biosynthesis downstream pathway, and the unknown bornyl pyrophosphate hydrolase may not be the specific enzyme for borneol formation. Additionally, the DXR, HDS, and IDS found in the PPI network would assist in the understanding of molecular regulation. The results of this study constructed DGE libraries and identified key genes related to camphane volatile terpenes, which laid a foundation for a deep investigation of the mechanism of volatile terpene biosynthesis, and provided a reference for mining other key genes in fruits.
()Lour.,该科的一种药用植物,用于医疗目的。乙酸龙脑酯、樟脑和冰片是该植物果实中的主要生物活性成分,乙酸龙脑酯的含量被视为果实品质的一个衡量指标。为了探究调控莰烷类挥发性萜生物合成的主要有效基因,本研究分析并总结了一些参与莰烷类挥发性萜生物合成的差异表达基因(DEGs)和转录因子。结果表明,乙酸龙脑酯的含量在不同生长阶段有所变化。特别是在授粉后7至30天(DAP)出现了显著变化。授粉后30天乙酸龙脑酯的含量比授粉后7天高出169.3%。在G2与G1的比较中,共发现4782个上调单基因和5284个下调单基因,在G3与G1的比较中,有3324个上调单基因和5
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