Li Shan, Jiang Yi, Xie Hanxiu, Wang Kangwei, Yang Kebang, Cao Qian, Xue Hao
School of Horticulture, Anhui Agricultural University, Hefei 230036, China.
Shanghai Agricultural Technology Extension Service Center, Shanghai 201103, China.
Plants (Basel). 2025 Jun 12;14(12):1804. doi: 10.3390/plants14121804.
Strawberry ( Duch.) is an important fruit worldwide whose growth, development, and productivity are threatened by salinity. The WRKY transcription factors (TFs) were reported to play an important role in regulating abiotic stresses response. However, research on their roles to regulate salt stress tolerance in strawberry remains limited. In current study, the gene was isolated and characterized from the Ruegen strawberry, and induced by various stress treatment. The results showed that the FvWRKY75 transcription factor was a transcriptional activator and localized in the nucleus. Phenotypic and physiological analysis revealed that ectopic expression of in improved salt tolerance by enhancing the antioxidant system activities, modulating ROS scavenging and upregulating stress-related genes. Y1H and dual luciferase assays revealed that FvWRKY75 can directly bind to the promoter of the gene by recognizing the W-box element. Compared with the WT, ectopic expression of gene in enhanced salt tolerance characterized by the reduced ROS accumulation, higher chlorophyll content, lower MDA content, and enhanced SOD and POD activity. Herein, the FvWRKY75 gene acted as a positive regulator in salt stress resistance, at least in part, via the WRKY-CRK network to regulate the antioxidant enzyme defense system and stress-related genes to regulate salt stress tolerance in strawberry.
草莓（Fragaria × ananassa Duch.）是一种在全球范围内重要的水果，其生长、发育和生产力受到盐胁迫的威胁。据报道，WRKY转录因子在调节非生物胁迫反应中起重要作用。然而，关于它们在调节草莓耐盐性方面的作用的研究仍然有限。在当前研究中，从鲁根草莓中分离并鉴定了该基因，并通过各种胁迫处理诱导。结果表明，FvWRKY75转录因子是一种转录激活因子，定位于细胞核。表型和生理分析表明，在拟南芥中异位表达FvWRKY75通过增强抗氧化系统活性、调节活性氧清除和上调胁迫相关基因来提高耐盐性。酵母单杂交和双荧光素酶测定表明，FvWRKY75可以通过识别W-box元件直接结合到CRK基因的启动子上。与野生型相比，在草莓中异位表达FvWRKY75基因增强了耐盐性，表现为活性氧积累减少、叶绿素含量更高、丙二醛含量更低以及超氧化物歧化酶和过氧化物酶活性增强。在此，FvWRKY75基因至少部分地通过WRKY-CRK网络作为盐胁迫抗性的正调控因子，以调节抗氧化酶防御系统和胁迫相关基因来调节草莓的耐盐性。
