Almeida Addison R, Pinheiro Francisco A S D, Fideles Marília G M, Cunha Roberto B L, Confessor Vitor P P, Matsui Kátia N, Paiva Weslley S, Rocha Hugo A O, Ganade Gislene, Espindola Laila S, Morais Waldenice A, Ferreira Leandro S
Programa de Pós-Graduação em Química, Instituto de Química, Universidade Federal do Rio Grande do Norte (UFRN), Natal CEP 59072-970, Brazil.
Programa de Pós-Graduação em Ciências Farmacêuticas, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte (UFRN), Natal CEP 59012-570, Brazil.
Pharmaceutics. 2025 Jun 14;17(6):780. doi: 10.3390/pharmaceutics17060780.
(Tul.) Gagnon & G.P. Lewis (Fabaceae), popularly known as "catingueira", is a plant widely distributed in the Caatinga biome, which comprises 11% of the Brazilian territory. While this species is of interest given local knowledge, formal reports are lacking in the literature, warranting targeted investigation. This study aimed to prepare and characterize a hydroethanolic extract of leaves, prepare carbopol gels containing the extract, and evaluate their cytotoxicity and antibacterial activity against and . : The initial extract was prepared in an ultrasonic bath using ethanol/water (70:30, /). The extract (1 mg/mL) was analyzed by liquid chromatography coupled with mass spectrometry (UHPLC-MS/MS). Carbopol-based gels containing 1% and 3% of extract were prepared and characterized in terms of pH, conductivity, spreadability, and rheology. The cytotoxicity was determined by the MTT method using MC3T3-E1 pre-osteoblast cells and L929-CCL1 fibroblast cells. The antibacterial activity of the extract and gels was evaluated using the agar diffusion method against and . : The leaves extract demonstrated antibacterial activity against and , were not cytotoxic for the assessed cells at concentrations up to 100 μg/mL, and its analysis by UHPLC-MS/MS allowed the annotation of 18 metabolites, mainly of the phenolic acid and flavonoids glycoside classes, together with a biflavonoid. The prepared gels remained stable over the 30-day post-production analysis period. : These findings provide a better understanding of the chemical diversity of the secondary metabolites of a common Caatinga biome species--specifically present in leaves hydroethanolic extract and gel formulation adapted for skin application with activity against
（图尔.）加尼翁和G.P.刘易斯（豆科），俗称“卡廷盖拉”，是一种广泛分布于卡廷加生物群落的植物，该生物群落占巴西领土的11%。尽管根据当地知识该物种具有研究价值，但文献中缺乏正式报道，因此有必要进行针对性研究。本研究旨在制备并表征叶的水乙醇提取物，制备含该提取物的卡波姆凝胶，并评估其对……的细胞毒性和抗菌活性。方法：初始提取物在超声浴中用乙醇/水（70:30，v/v）制备。提取物（1mg/mL）通过液相色谱-质谱联用（UHPLC-MS/MS）进行分析。制备了含1%和3%提取物的卡波姆基凝胶，并对其pH值、电导率、铺展性和流变学进行了表征。细胞毒性通过MTT法使用MC3T3-E1前成骨细胞和L929-CCL1成纤维细胞进行测定。提取物和凝胶的抗菌活性通过琼脂扩散法针对……进行评估。结果：叶提取物对……表现出抗菌活性，在浓度高达100μg/mL时对所评估细胞无细胞毒性，通过UHPLC-MS/MS分析可鉴定出18种代谢物，主要为酚酸和黄酮苷类，以及一种双黄酮。制备的凝胶在生产后30天的分析期内保持稳定。结论：这些发现有助于更好地了解卡廷加生物群落常见物种次生代谢物的化学多样性——具体存在于叶水乙醇提取物和适用于皮肤应用且具有活性的凝胶制剂中。
