Gao Yuxin, Jiang Dongqi, Wang Mengying, Du Xueqing, Zong Hong, Zhuge Bin
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Lab of Industrial Microorganism & Research and Design Center for Polyols, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Biotechnol Lett. 2025 Jun 27;47(4):68. doi: 10.1007/s10529-025-03602-7.
Expressing cellulase systems in C. glycerinogenes with extracellular secretion ability, and using fermentation with residue, enabled the recombinant strain to degrade lignocellulosic waste for efficient glycerol production, offering new option for agricultural waste transformation.
Candida glycerinogenes is employed as the host strain, various cellulases were screened. Signal peptide mining and screening, and semi-rational design strategy was adopted in the host strain. The recombinant strain Cg4 had a total cellulase activity of 13.6 U/mL. Cg4 was applied to 110 g/L sugarcane bagasse hydrolysate with 40 g/L of pretreated bagasse as the substrate. The glycerol yield reached 50 g/L, with a 43.3% bagasse utilization rate, the amount of cellulase used in the degradation of lignocellulose was reduced by 12.1%.
Opened up a route for efficient degradation of agricultural waste of lignocellulose by C. glycerinogenes for glycerol production, the emission of lignocellulose waste had been reduced, and decreasing the need for cellulases in hydrolysis.
在具有胞外分泌能力的甘油克鲁维酵母中表达纤维素酶系统，并利用残渣进行发酵，使重组菌株能够降解木质纤维素废料以高效生产甘油，为农业废弃物转化提供新选择。
以甘油假丝酵母作为宿主菌株，筛选了多种纤维素酶。在宿主菌株中采用信号肽挖掘与筛选以及半理性设计策略。重组菌株Cg4的总纤维素酶活性为13.6 U/mL。将Cg4应用于以40 g/L预处理甘蔗渣为底物的110 g/L甘蔗渣水解液中。甘油产量达到50 g/L，甘蔗渣利用率为43.3%，木质纤维素降解中纤维素酶的用量减少了12.1%。
开辟了一条利用甘油克鲁维酵母高效降解农业木质纤维素废料生产甘油的途径，减少了木质纤维素废料的排放，并降低了水解过程中对纤维素酶的需求。
