Qin Zhao, Wang Dan, Zhang Ruilai, Lin Fanzhen, Wang Mengdie, Hu Lin, Kang Yaqi, Li Wenwei
School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China.
School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China.
Int J Biol Macromol. 2025 Aug;320(Pt 3):146026. doi: 10.1016/j.ijbiomac.2025.146026. Epub 2025 Jul 14.
The production of high-value succinic acid (SA) from waste jatropha fruit shell (JFS) lignocellulose biomass is considered to be an economical and environmentally friendly strategy. Firstly, the cellulase encapsulated in liposomes was used to construct an efficient enzyme-catalyzed microenvironment. Furthermore, the hydrolysis efficiency of JFS was enhanced by simultaneously activating lignocellulose and in-situ saccharification in ionic liquid (IL). Finally, carbonic anhydrase and phosphoenolpyruvate carboxylase were co-expressed in Escherichia coli to produce SA efficiently from JFS hydrolysate. The results indicated that the enzymatic activity could be maintained at 90.5 % and the total reducing sugar yield was as high as 80.2 % in cellulase-containing liposomes at low temperature of 50 °C for 24 h. In addition, 36.1 g/L SA with a yield of 0.92 g/g could be produced by using JFS hydrolysate. Biocompatible IL choline proline enhance the decomposition of lignocellulose through strong hydrogen bonding, electrostatic mismatch and covalent coupling with lignocellulose and liposomes, and liposomes promote in-situ enzymatic hydrolysis of lignocellulose through the high-energy catalytic conformation of enzymes in a confined space. This work demonstrates the green synthesis of high-value SA using JFS hydrolysate as a feedstock, which holds potential for applications in the conversion of waste biomass into chemical products.
利用麻风树果壳（JFS）木质纤维素生物质生产高价值琥珀酸（SA）被认为是一种经济且环保的策略。首先，将包封在脂质体中的纤维素酶用于构建高效的酶催化微环境。此外，通过在离子液体（IL）中同时激活木质纤维素和原位糖化来提高JFS的水解效率。最后，在大肠杆菌中共表达碳酸酐酶和磷酸烯醇式丙酮酸羧化酶，以从JFS水解产物中高效生产SA。结果表明，在50℃低温下24小时，含纤维素酶的脂质体中的酶活性可维持在90.5%，总还原糖产率高达80.2%。此外，使用JFS水解产物可生产出36.1 g/L的SA，产率为0.92 g/g。生物相容性离子液体胆碱脯氨酸通过与木质纤维素和脂质体形成强氢键、静电失配和共价偶联来增强木质纤维素的分解，而脂质体通过在受限空间内酶的高能催化构象促进木质纤维素的原位酶水解。这项工作展示了以JFS水解产物为原料绿色合成高价值SA，在将废弃生物质转化为化学产品方面具有应用潜力。
