Giraud Romain, Le Blanc J C Yves, Guna Mircea, Hopfgartner Gérard
Life Sciences Mass Spectrometry, Department of Inorganic and Analytical Chemistry, University of Geneva, 24 Quai Ernest Ansermet, Geneva 4 CH-1205, Switzerland.
SCIEX, Toronto, Ontario M4B 1G2, Canada.
Anal Chem. 2025 Jul 15;97(27):14503-14511. doi: 10.1021/acs.analchem.5c01831. Epub 2025 Jun 27.
A commercial QqTOF platform (ZenoTOF 7600 system) was modified to enable three fragmentation modes: collision-induced dissociation (CID), electron-activated dissociation (EAD), and ultraviolet photodissociation (UVPD) at 266 nm. 168 pesticides, which showed fragmentation in CID, also provide EAD spectra. In the case of UVPD, 158 compounds fragmented under 266 nm photon irradiation. The performance of the novel platform was evaluated using data-independent SWATH CID acquisition for the general screening, and multiple product ion acquisitions were scheduled with CID/EAD/UVPD for confirmatory analysis of pesticides in juice and white and red wine samples. A column-switching liquid chromatography (LC) method with online dilution was developed to inject large volumes (80 μL) into the system. The approach enabled the detection and concentration estimation of approximately 30 pesticides in juices and wines, including insecticides, neonicotinoids, and fungicides. Pesticide limits of detection (LODs) were found to be in the picogram per milliliter to nanogram per milliliter range for MS1 and MS2 acquisitions.
一个商用的四极杆飞行时间（QqTOF）平台（ZenoTOF 7600系统）经过改造，以实现三种碎裂模式：碰撞诱导解离（CID）、电子激活解离（EAD）和266nm的紫外光解离（UVPD）。168种在CID中表现出碎裂的农药，也能提供EAD光谱。在UVPD的情况下，158种化合物在266nm光子照射下发生碎裂。使用数据非依赖型SWATH CID采集对该新型平台的性能进行了评估，以进行常规筛查，并安排使用CID/EAD/UVPD进行多产物离子采集，用于果汁、白葡萄酒和红葡萄酒样品中农药的确证分析。开发了一种带在线稀释的柱切换液相色谱（LC）方法，以便向系统中注入大量（80μL）样品。该方法能够检测和估算果汁和葡萄酒中约30种农药的浓度，包括杀虫剂、新烟碱类和杀菌剂。对于MS1和MS2采集，农药的检测限（LOD）在皮克每毫升至纳克每毫升范围内。
