Mosse P R, Campbell G R, Wang Z L, Campbell J H
Lab Invest. 1985 Nov;53(5):556-62.
The volume fraction of synthetic organelles in smooth muscle cells from diffuse intimal thickenings (DIT) and media of human carotid arteries has been determined morphometrically. Fresh tissue from surgical endarterectomy was obtained from both male and female patients between 50 and 80 years of age. The results show that 87% of cells within the DIT can be readily identified as smooth muscle. The volume fraction of synthetic organelles in smooth muscle cells from the DIT of both males and females is of a highly significant difference from the volume fraction of synthetic organelles in cells of the subjacent media. This represents a 2-fold increase in the content of synthetic organelles in the cells of the DIT. The results also show greater phenotypic variability among cells of the DIT than among cells of the media. The results are discussed in the context of smooth muscle cell phenotypic changes in cell culture and the implications of such changes in phenotypic expression in the initiation of atherogenesis.
已通过形态计量学确定了来自人类颈动脉弥漫性内膜增厚（DIT）和平滑肌层的平滑肌细胞中合成细胞器的体积分数。取自50至80岁男性和女性患者的外科动脉内膜切除术新鲜组织。结果表明，DIT内87%的细胞可轻易鉴定为平滑肌细胞。男性和女性DIT平滑肌细胞中合成细胞器的体积分数与相邻平滑肌层细胞中合成细胞器的体积分数存在高度显著差异。这表明DIT细胞中合成细胞器的含量增加了两倍。结果还表明，DIT细胞之间的表型变异性比平滑肌层细胞之间更大。本文结合细胞培养中平滑肌细胞的表型变化以及这些表型变化在动脉粥样硬化发生起始中对表型表达的影响进行了讨论。
