Han Tong, Cui Yongmei, Jing Yang, Liu Meiying, Chen Xuanchao, Song Yinghui, Gu Xinping, Wang Jiahao, Wang Long
Shandong Provincial Key Laboratory of Biochemistry and Molecular Biology, School of Advanced Agricultural Sciences, Weifang University, Weifang, China.
Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Agriculture and Forestry Academy, Qinghai University, Xining, China.
Front Plant Sci. 2025 Jun 13;16:1607751. doi: 10.3389/fpls.2025.1607751. eCollection 2025.
, an important root vegetable native to China, is widely cultivated for its nutritional value and diverse applications. However, it is highly sensitive to lead (Pb) stress, with Pb predominantly accumulating in the roots. Previous studies have highlighted the pivotal role of xyloglucan endotransglucosylase/hydrolase (XTH) in plant responses to heavy metal stress. Despite this, a comprehensive identification, molecular characterization, and functional analysis of the gene family in has been lacking. In this study, 28 genes were identified in . Gene structure analysis revealed the presence of eight conserved motifs, along with variations in exon-intron organization and chromosomal distribution across all chromosomes. Phylogenetic analysis of genes from , and grouped them into five distinct clades, suggesting their evolutionary conservation and potential functional diversification. Transcriptome sequencing and qRT-PCR analysis showed that was strongly induced by Pb stress. Transgenic hairy roots overexpressing exhibited enhanced Pb tolerance, evidenced by reduced chlorosis, increased fresh weight, improved photosynthetic performance, and lower oxidative damage under Pb stress. Furthermore, several transcription factors, such as RsERF2, RsHD-ZIP22, and etc., exhibited strong positive correlations with , implying their roles in regulating Pb-induced expression. Overall, this study provides insights into the gene family in and highlights their critical roles in Pb stress response.
[某种植物名称]，一种原产于中国的重要根茎类蔬菜，因其营养价值和多样用途而被广泛种植。然而，它对铅（Pb）胁迫高度敏感，铅主要在根部积累。先前的研究强调了木葡聚糖内转糖基酶/水解酶（XTH）在植物对重金属胁迫反应中的关键作用。尽管如此，对[该植物名称]中[XTH]基因家族的全面鉴定、分子特征分析和功能分析仍属空白。在本研究中，在[该植物名称]中鉴定出了28个[XTH]基因。基因结构分析揭示了8个保守基序的存在，以及外显子-内含子组织和染色体分布在所有染色体上的变化。对来自[该植物名称]、[另一植物名称]和[又一植物名称]的[XTH]基因进行的系统发育分析将它们分为五个不同的进化枝，表明它们在进化上的保守性和潜在的功能多样化。转录组测序和qRT-PCR分析表明，[XTH基因名称]在铅胁迫下被强烈诱导。过表达[XTH基因名称]的转基因毛状根表现出增强的铅耐受性，在铅胁迫下，叶片褪绿减少、鲜重增加、光合性能改善以及氧化损伤降低都证明了这一点。此外，几个转录因子，如RsERF2、RsHD-ZIP22等，与[XTH基因名称]表现出强烈的正相关，暗示它们在调节铅诱导的[XTH基因名称]表达中的作用。总体而言，本研究为[该植物名称]中的[XTH]基因家族提供了见解，并突出了它们在铅胁迫反应中的关键作用。
