Wang Xiaobing, Yao Jingting, Wang Xiaotong, Yang Fan, Zhao Tanyan, Wang Hongbo, Li Nan, Ko H M U U T H U K O, Oo K Y A W Z I N, Jiang Luyao, Gao Song, Huan Changchao
Institute of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China; Key Laboratory of Avian Bioproduct Development, Ministry of Agriculture and Rural Affairs, Yangzhou, China.
Institute of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China; Key Laboratory of Avian Bioproduct Development, Ministry of Agriculture and Rural Affairs, Yangzhou, China.
Int J Biol Macromol. 2025 May;306(Pt 2):141158. doi: 10.1016/j.ijbiomac.2025.141158. Epub 2025 Feb 16.
Pseudorabies virus (PRV) is an alpha herpes virus that causes pseudorabies. Pigs are the only natural hosts of the virus. This disease has caused considerable economic losses to the global pig industry. Since 2011, the effectiveness of vaccines has decreased greatly because of the emergence of various PRV varieties. Additionally, some people are infected with PRV. Therefore, the development of new anti-PRV agents is highly important. Lysimachia christinae is a traditional Chinese herbal medicine that has favorable effects on urine, detoxification, and swelling. In this study, the in vitro and in vivo anti-PRV activities of Lysimachia christinae polysaccharide (LCP) were investigated. Our results revealed that 200 μg/mL LCP significantly attenuated the infection of PK-15 B6 cells by PRV XJ5 and that 100 mg/kg LCP significantly reduced PRV XJ5 infection in mice. Mechanistic studies revealed that the main target of the anti-PRV effect of LCP was in the virus adsorption stage. In addition, our study revealed that LCP can reduce reactive oxygen species (ROS) and malondialdehyde (MDA) levels in PRV XJ5-infected PK-15 B6 cells and increase the activity of superoxide dismutase (SOD). These results showed that LCP can reduce the oxidative stress caused by PRV infection. In summary, LCP may be used as an antiviral drug to fight new PRV mutant strains.
伪狂犬病病毒（PRV）是一种引起伪狂犬病的甲型疱疹病毒。猪是该病毒唯一的天然宿主。这种疾病给全球养猪业造成了巨大的经济损失。自2011年以来，由于各种PRV变种的出现，疫苗的效力大幅下降。此外，一些人也感染了PRV。因此，开发新的抗PRV药物非常重要。金钱草是一种对利尿、解毒和消肿有良好效果的传统中草药。在本研究中，对金钱草多糖（LCP）的体外和体内抗PRV活性进行了研究。我们的结果显示，200μg/mL的LCP显著减轻了PRV XJ5对PK-15 B6细胞的感染，100mg/kg的LCP显著降低了小鼠体内PRV XJ5的感染。机制研究表明，LCP抗PRV作用的主要靶点在病毒吸附阶段。此外，我们的研究还显示，LCP可以降低PRV XJ5感染的PK-15 B6细胞中的活性氧（ROS）和丙二醛（MDA）水平，并提高超氧化物歧化酶（SOD）的活性。这些结果表明，LCP可以减轻PRV感染引起的氧化应激。综上所述，LCP可能用作对抗新的PRV突变株的抗病毒药物。
