Wang Chun-Zheng, Dong Jia-Nan, Sun Wu-Wen, Qu Ke, Lin Zi-Chen, Aschalew Natnael D, Zhao Yang, Sun Zhe, Ta Na, Zhao Zhi-Kun, Liu Yong-Jun, Zhang Wei-Gang, Zhao Wei, Qin Gui-Xin, Zhen Yu-Guo, Wang Tao
College of Animal Science and Technology, Jilin Agricultural University-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, Key Laboratory of Animal Production Product Quality and Security Ministry of Education, Jilin Agricultural University, Changchun, China.
College of Animal Science and Technology, Ningxia University, Yinchuan, China.
Front Immunol. 2025 Jun 16;16:1605244. doi: 10.3389/fimmu.2025.1605244. eCollection 2025.
Yeast culture (YC) has become a crucial microecological preparation for regulating the ruminal environment and improving ruminant health. -inositol is an effective substance in YC, our study aimed to investigate whether -inositol can regulate metabolism and influence the health of dairy cows. Therefore, this study explored the effects of -inositol on the health, metabolome, and lactation performance of peripartum dairy cows using both and models.
The ANKOM RFS gas production system was used for the experiment; Twenty four healthy Holstein cows were assigned to CON group (n = 4; no supplementation of YC or -inositol), YC group (n = 10; 2 g/kg YC), and IN65 group (n = 10; 2 g/kg YC + 16 mg/kg -inositol) for experiment.
The concentrations of five volatile fatty acids (acetic acid, butyric acid, isobutyric acid, valeric acid, and isovaleric acid), and total volatile fatty acids were significantly higher in the IN65 group compared to the CON group at 6 h ( < 0.01). Therefore, 2 g/kg YC plus 16 mg/kg -inositol was selected for experiment. It was found that the average milk production of the IN65 group increased by 4.00% and 4.05% respectively, compared to the control group and YC group on day 21 postpartum. The levels of superoxide dismutase (SOD), total antioxidant status (TAS), and total oxidative status (TOS) were significantly higher on day 21 compared to day 1 postpartum ( < 0.01). However, a trend indicating a decrease in immune function was observed in the IN65 group compared to the CON group. These findings suggested that -inositol may improve the health of postpartum dairy cows. Additionally, Mantel analysis showed acetic acid was positively correlated with D-glyceric acid and xanthosine. Similarly, D-glyceric acid, inosine 5-monophosphate, and xanthosine were positively correlated with microbiota at the genus, such as and .
In summary, -inositol influences energy metabolism by modulating rumen microbial abundance, improving dairy cow health, and increasing milk production.
酵母培养物（YC）已成为调节瘤胃环境和改善反刍动物健康的关键微生态制剂。肌醇是YC中的一种有效物质，我们的研究旨在调查肌醇是否能调节代谢并影响奶牛健康。因此，本研究使用体内和体外模型探讨了肌醇对围产期奶牛健康、代谢组和泌乳性能的影响。
ANKOM RFS气体产生系统用于体外实验；24头健康的荷斯坦奶牛被分配到对照组（n = 4；不补充YC或肌醇）、YC组（n = 10；2 g/kg YC）和IN65组（n = 10；2 g/kg YC + 16 mg/kg肌醇）进行体外实验。
在6小时时，IN65组的五种挥发性脂肪酸（乙酸、丁酸、异丁酸、戊酸和异戊酸）以及总挥发性脂肪酸的浓度显著高于对照组（P < 0.01）。因此，选择2 g/kg YC加16 mg/kg肌醇进行体内实验。结果发现，产后第21天，IN65组的平均产奶量分别比对照组和YC组提高了4.00%和4.05%。与产后第1天相比，第21天超氧化物歧化酶（SOD）、总抗氧化状态（TAS）和总氧化状态（TOS）水平显著升高（P < 0.01）。然而，与对照组相比，IN65组观察到免疫功能有下降趋势。这些发现表明肌醇可能改善产后奶牛的健康。此外，Mantel分析表明乙酸与D-甘油酸和黄苷呈正相关。同样，D-甘油酸、5'-肌苷酸和黄苷与属水平的微生物群呈正相关，如普雷沃氏菌属和瘤胃球菌属。
总之，肌醇通过调节瘤胃微生物丰度、改善奶牛健康和提高产奶量来影响能量代谢。
