Sun Yifan, Li Xiao, Dong Yan, Zhang Xiaoli, Gan Ling, Jia Tianming
Department of Children's Development and Behavior Center, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Department of Pediatric Neurology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Front Neurol. 2025 Jun 16;16:1587200. doi: 10.3389/fneur.2025.1587200. eCollection 2025.
To investigate the effect of visual stimulation on epilepsy susceptibility in neonatal hypoglycemic brain injury (HBIN) rats and its underlying mechanisms.
Seventy-five 2-day-old Sprague-Dawley rats were divided into three groups: control (N, = 25), model (NH, = 25), and visual stimulation (NH-V, = 25). The NH and NH-V groups were injected with insulin (40 U/kg) on postnatal days 2, 4, and 6, and blood glucose was monitored. The NH-V group received daily 2-h visual stimulation from P14 to P28. At P21, brain Magnetic Resonance Imaging (MRI) was performed. Pentylenetetrazol (PTZ) was injected to induce seizures and recorded at P28. Brain tissue was analyzed for Brain-Derived Neurotrophic Factor (BDNF) and Synaptophysin (SYN) expression.
(i) In the NH and NH-V groups, blood glucose decreased after insulin injection, with behavioral changes observed at 1-4 h. One rat in the NH group had spontaneous seizures. (ii) MRI at 15 days showed occipital lobe abnormalities in 50% of NH rats, with no changes in controls. (iii) In PTZ-induced seizures, the N group had significantly lower seizure scores than the NH group, with the NH-V group showing further reduction. (iv) BDNF and SYN expression were higher in the NH-V group compared to the NH group.
Visual stimulation reduces epilepsy susceptibility in neonatal HBIN rats, likely through upregulation of BDNF and SYN expression in the occipital cortex.
探讨视觉刺激对新生低血糖脑损伤（HBIN）大鼠癫痫易感性的影响及其潜在机制。
将75只2日龄的Sprague-Dawley大鼠分为三组：对照组（N，=25）、模型组（NH，=25）和视觉刺激组（NH-V，=25）。NH组和NH-V组在出生后第2、4和6天注射胰岛素（40 U/kg），并监测血糖。NH-V组从P14至P28每天接受2小时的视觉刺激。在P21时进行脑磁共振成像（MRI）。注射戊四氮（PTZ）诱导癫痫发作，并在P28时记录。分析脑组织中脑源性神经营养因子（BDNF）和突触素（SYN）的表达。
（i）在NH组和NH-V组中，注射胰岛素后血糖降低，在1-4小时观察到行为变化。NH组中有1只大鼠出现自发性癫痫发作。（ii）15天时的MRI显示，50%的NH大鼠枕叶有异常，对照组无变化。（iii）在PTZ诱导的癫痫发作中，N组的癫痫发作评分显著低于NH组，NH-V组进一步降低。（iv）与NH组相比，NH-V组中BDNF和SYN的表达更高。
视觉刺激可降低新生HBIN大鼠的癫痫易感性，可能是通过上调枕叶皮质中BDNF和SYN的表达来实现的。
