Saito Y, Ozawa T, Hayashi H, Nishiyama A
Pflugers Arch. 1985 Sep;405(2):108-11. doi: 10.1007/BF00584530.
Using double-barreled Cl- -sensitive microelectrodes, intracellular Cl- activity (AiCl) in the mouse lacrimal acinar cells in vitro was determined in both resting and secretory phases. In the resting state AiCl was 31 mmol/l which was 1.4 times higher than that predicted for the passive distribution according to the membrane potential (Vm) of -41 mV. Addition of acetylcholine (ACh, 1 microM) hyperpolarized Vm to -63 mV and decreased AiCl to 20 mmol/l which was still twice the equilibrium activity. A-23178 produced similar changes in Vm and AiCl to those induced by ACh. It was concluded that Cl- was actively accumulated in the acinar cells and, in the secretory phase, Cl- efflux was enhanced by the increased driving force and Ca2+-mediated increase in the Cl- permeability across the cell membrane.
使用双管 Cl⁻ 敏感微电极，在体外测定小鼠泪腺腺泡细胞在静息期和分泌期的细胞内 Cl⁻ 活性（AiCl）。在静息状态下，AiCl 为 31 mmol/L，比根据 -41 mV 的膜电位（Vm）预测的被动分布值高 1.4 倍。添加乙酰胆碱（ACh，1 μM）使 Vm 超极化至 -63 mV，并使 AiCl 降至 20 mmol/L，这仍为平衡活性的两倍。A-23178 对 Vm 和 AiCl 产生的变化与 ACh 诱导的变化相似。得出的结论是，Cl⁻ 在腺泡细胞中被主动积累，并且在分泌期，Cl⁻ 外流因驱动力增加和 Ca²⁺ 介导的细胞膜 Cl⁻ 通透性增加而增强。
